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首页> 外文期刊>Archives of microbiology >Structural modeling and environmental regulation of arginine decarboxylase in Synechocystis sp PCC 6803
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Structural modeling and environmental regulation of arginine decarboxylase in Synechocystis sp PCC 6803

机译：Synechocystis SPCC 6803中精氨酸脱羧酶的结构建模与环境调控

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摘要

Arginine decarboxylase (ADC) is the first enzyme in the alternative route to putrescine in the polyamine biosynthesis pathway in bacteria and plants. In this study, we have focused on the effects of various types of short-term stresses on the transcript amount and specific activity of Synechocystis sp. PCC 6803 ADC. Our results reveal that the steady-state transcript accumulation and enzyme activity are not connected in a simple manner, since only photoheterotrophy and synergistic salt and high-light stress affected both parameters similarly. Changes in the steady-state ADC mRNA accumulation under the other short-term stress conditions studied had only a small impact on enzyme activity, suggesting post-translational regulation. Based on structural modeling, Synechocystis ADCs have a putative extra domain, which might be involved in the post-translational regulation of ADC activity in Synechocystis. In addition, two symmetric inter-subunit disulfide bonds seem to stabilize the dimeric structure of ADCs. There are two genes coding for ADC and agmatinase, another polyamine pathway enzyme, in Synechocystis genome, while the genes coding for ornithine decarboxylase and for some other enzymes in the polyamine pathway were not identified with homology searches.
机译：精氨酸脱羧酶（ADC）是在细菌和植物中的多胺生物合成途径中腐败的替代途径中的第一种酶。在这项研究中，我们专注于各种类型的短期应力对综合症SP的转录量和特异性活性的影响。 PCC 6803 ADC。我们的结果表明，稳态转录物积累和酶活性未以简单的方式连接，因为只有摄影术和协同盐和高光应力同样影响了两个参数。在研究的其他短期应激条件下，稳态ADC mRNA积累的变化仅对酶活性进行了少量影响，表明翻译后调节。基于结构建模，SyneChocystis ADC具有推定的额外域，其可能参与综合症中ADC活性的翻译后调节。此外，两个对称的亚基二硫化物键似乎稳定ADC的二聚体结构。在SyneChocystis基因组中，存在两种基因和Adamatinase，另一种聚酰胺途径酶，同时编码鸟氨酸脱羧酶的基因和在多胺途径中的一些其他酶的基因未与同源性搜索鉴定。

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