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首页> 外文期刊>Archives of microbiology >Staphylococcus enterotoxin profile of China isolates and the superantigenicity of some novel enterotoxins
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Staphylococcus enterotoxin profile of China isolates and the superantigenicity of some novel enterotoxins

机译：葡萄球菌肠毒素的中国分离物和一些新肠毒素的超人物

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摘要

The genus of staphylococcus widely distributes in environments and contributes to a variety of animal and human diseases. The enterotoxins (SEs) secreted by this type of pathogen have been the leading cause of bacterial toxic shock syndrome and food poisoning, and thus present a substantial concern to public health. In this study, we analyzed the superantigen profile of 122 staphylococcus strains isolated from diverse sources. When screened for the presence and prevalence of 17 known se or se-like (sel) genes, except selj, all other genes were detected in these isolates. In particular, 95.9% of the isolates harbored at least one se/sel gene. Moreover, 47.5% of them bore at least 5. Remarkably, several non-pathogenic species of animal- and environment-origin were also found to carry multiple se/sels. The most frequent genes detected were tsst (62.3%), sei (54.1%), and seb (46.7%), followed by some sel genes (selo, selu, and selm), which also were present at relatively high frequency (20-30%). The generated data improved understanding of strain-specific differences in enterotoxin expression. The gene products of the latter (selo and selu) were subsequently analyzed for their antigenicity in a mouse model using purified E. coli-based recombinant proteins. The studies revealed a strong activity for SEO in induction of T-lymphocyte proliferation and production of various inflammatory cytokines either in vivo or in vitro. In contrast, SEU exhibited little superantigenic effects. The molecular basis for the difference in antigenicity was analyzed by 3D homology remodeling, which revealed a difference in binding and affinities for MHC-II molecules and TCR V beta region.
机译：金葡萄球菌属广泛分布在环境中，有助于各种动物和人类疾病。通过这种类型的病原体分泌的肠毒素（SES）是细菌毒性休克综合征和食物中毒的主要原因，因此对公共卫生提出了重大关注。在这项研究中，我们分析了从不同来源分离的122个葡萄球菌菌株的超抗原谱。当筛选出现17种已知的SE或SE样（SEL）基因的存在和患病率，除了SELJ之外，在这些分离株中检测到所有其他基因。特别地，95.9％的分离物覆有至少一个SE / SEL基因。此外，其中47.5％的孔至少为5.显着，还发现，几种非致病性动物和环境起源也被发现携带多种SE / SEL。检测到最常见的基因是TSST（62.3％），SEI（54.1％）和SEB（46.7％），其次是一些SEL基因（SELO，SELU和SELM），其也以相对高的频率存在（20- 30％）。产生的数据改善了对肠毒素表达中应变特异性差异的理解。随后使用纯化的大肠杆菌基重组蛋白在小鼠模型中分析后一种（SELO和苏尔和苏尔氏）的基因产物。研究表明，SEO在体内或体外诱导T淋巴细胞增殖和各种炎症细胞因子的产生的强烈活动。相比之下，SEU表现出少量的超毒性效果。通过3D同源重塑分析抗原性差异的分子基础，这揭示了MHC-II分子和TCRVβ区域的结合和亲和力的差异。

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