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Frankia discariae sp nov.: an infective and effective microsymbiont isolated from the root nodule of Discaria trinervis

机译:Frankia Discariae SP Nov.:从迪斯科耳术的根结节中分离出一种感染性和有效的微腺

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Strain BCU110501(T) was the first isolate reported to fulfill Koch's postulates by inducing effective nodules on its host plant of origin Discaria trinervis (Rhalmnaceae). Based on 16S rRNA gene sequence similarities, the strain was found to be most closely related to the type strain of Frankia elaeagni DSM 46783(T) (98.6%) followed by F. alni DSM 45986(T) (98.2%), F. casuarinae DSM 45818(T) (97.8%) and F. inefficacies DSM 45817(T) (97.8%). Digital DNA:DNA hybridizations (dDDH) between strain BCU110501(T)and the type strains of other Frankia species were clearly below the cutoff point of 70%. The G+C content of DNA is 72.36%. The cell wall of strain BCU110501(T) contained meso-diaminopimelic acid and the cell sugars were galactose, glucose, mannose, xylose and ribose. Polar lipids were phosphatidylinositol (PI), diphosphatidylglycerol (DPG), glycophospholipid (GPL(1-3)), phosphatidylglycerol (PG) and an unknown lipid (L). The major fatty acids of strain BCU110501(T) consisted of iso-C16:0, C17:1 w8c and C16:0. Major menaquinones were MK9 (H-4), MK9 (H-6) and MK9 (H-2). Based on these analyses, strain BCU110501(T) (=DSM 46785(T)=CECT 9042(T)) should be classified as the type strain of a novel Frankia species, for which the name Frankia discariae sp. nov. is proposed.
机译:菌株BCU110501(T)是据报道的第一种分离物,以促进其原产地浮动植物(Rhalmnaceae)的宿主植物上的有效结节。基于16S rRNA基因序列相似性,发现该菌株与弗兰基亚伊氏菌DSM 46783(T)(98.6%)的菌株最密切相关,然后是F. Alni DSM 45986(T)(98.2%),F。 Casuarinae DSM 45818(T)(97.8%)和F.效率效率DSM 45817(T)(97.8%)。数码DNA:菌株BCU110501(T)之间的DNA杂交(DDDH)和其他弗兰基岛物种的菌株明显低于70%的截止点。 DNA的G + C含量为72.36%。菌株BCU110501(T)的细胞壁含有甲基二氨基甲酸,细胞糖是半乳糖,葡萄糖,甘露糖,木糖和核糖。极性脂质是磷脂酰肌醇(PI),二磷脂酰甘油(DPG),糖基磷脂(GPL(1-3)),磷脂酰甘油(PG)和未知的脂质(L)。菌株BCU110501(T)的主要脂肪酸由ISO-C16:0,C17:1W8C和C16:0组成。主要的母牛是MK9(H-4),MK9(H-6)和MK9(H-2)。基于这些分析,应变BCU110501(T)(= DSM 46785(T)= CECT 9042(T))应被归类为新颖的弗兰克乃西物种的类型,为弗兰科·迪斯兰人SP的名称。 11月。提出。

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