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首页> 外文期刊>Archives of microbiology >The effect of reconstruction works on planktonic bacterial diversity of a unique thermal lake revealed by cultivation, molecular cloning and next generation sequencing
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The effect of reconstruction works on planktonic bacterial diversity of a unique thermal lake revealed by cultivation, molecular cloning and next generation sequencing

机译：重建工程对培养，分子克隆和下一代测序揭示的独特热湖中浮游细菌多样性的影响

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摘要

The aim of this study was to gain detailed information about the diversity of planktonic bacterial communities of a worldwide special peat bedded natural thermal spa lake, and to reveal the effect of a lake wall reconstruction work. To compare the efficiency of different methods used for analyzing bacterial diversity, cultivation, molecular cloning and pyrosequencing were applied simultaneously. Despite the almost unchanged physical-chemical parameters and cell count values of lake water, remarkable differences were observed in the planktonic bacterial community structures during and after the reconstruction by all applied microbiological approaches. Rhodobacter sp. was found to be one of the most abundant community members during the works probably due to the sediment stirring effect of the reconstruction. Following the reconstruction higher diversity was detected than during the works by all approaches. Bacterial strains related to species Chryseobacterium and Exiguobacterium, furthermore sequences related to Arcobacter, Gemmobacter and MWH-UniP1 aquatic group were identified in the highest proportion at that time. Although the differences revealed by cultivation based and independent community structures were significant, only minor disparities were found by molecular cloning and next generation sequencing techniques.
机译：本研究的目的是获得有关全球特殊泥炭玻璃天然热水器湖的浮游细菌群落的多样性的详细信息，并揭示了湖墙重建工作的效果。为了比较用于分析细菌多样性的不同方法的效率，同时施用培养，培养，分子克隆和焦磷酸盐。尽管存在湖水的几乎不变的物理化学参数和细胞计数，但在重建的所有应用的微生物方法中，在浮游细菌群落结构中观察到显着差异。乳杆菌sp。被发现是作品中最丰富的社区成员之一可能是由于重建的沉积物搅拌效果。在重建之后，检测到更高的多样性，而不是所有方法的工程。与物种菊杆菌和外杆菌有关的细菌菌株，此外与当时的最高比例鉴定了与Arcobacter，Gemmobacter和MWh-Unip1水生组相关的序列。虽然基于培养和独立群落结构的差异显着，但仅通过分子克隆和下一代测序技术发现了微小的差异。

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