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Identification of the zinc, copper and cadmium metalloproteome of the protozoon Tetrahymena thermophila by systematic bioinformatics

机译:通过系统生物信息学通过系统生物信息学原子液硫化物嗜热噬菌体锌,铜和镉金属蛋白酶鉴定

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摘要

Tetrahymena thermophila (T. thermophila) is a ciliated protozoon that can detect freshwater pollution by heavy metals ("whole-cell biosensor"). This work employed a systematic bioinformatics approach to predict and analyze the metalloproteome of T. thermophila for the essential Zn, Cu and the non-essential Cd. 3784 metal-binding proteins were identified compared to the 456 annotated so far in UniProt. The localization, functional classification, and the functionally enriched network of the newly identified metalloproteome are presented. Cd toxicity could be explained in terms of the metal replacing Cu and especially Zn in MAPKs, transporters and antioxidant enzymes. The predicted results for Cd toxicity and responses reflect those observed experimentally in different organisms after their exposure to Cd.
机译:Tetrahymena Hotherophila(T.Vircophila)是一种纤毛的原生液,可以通过重金属(“全细胞生物传感器”)检测淡水污染。 这项工作采用了系统的生物信息学方法来预测和分析必需Zn,Cu和非必需CD的T.Grothophila的金属蛋白。 将3784鉴定金属结合蛋白与迄今为止在UniProt的456次鉴定。 提出了新鉴定的金属蛋白组的定位,功能分类和功能富集网络。 可以在替代Cu,尤其是Mapks,运输术和抗氧化酶中的金属替换Cd,尤其是Zn的毒性。 CD毒性和反应的预测结果反映了在暴露于CD后在不同生物中观察的人。

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