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首页> 外文期刊>Archives of microbiology >Bradyrhizobium brasilense sp nov., a symbiotic nitrogen-fixing bacterium isolated from Brazilian tropical soils
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Bradyrhizobium brasilense sp nov., a symbiotic nitrogen-fixing bacterium isolated from Brazilian tropical soils

机译：Bradyrhizobium Brasilense SP 11月。，从巴西热带土壤中分离的共生氮素固定细菌

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摘要

Four strains of rhizobia isolated from nodules of Vigna unguiculata (UFLA03-321(T), UFLA03-320 and UFLA03-290) and Macroptilium atropurpureum (UFLA04-0212) in Brazilian soils were previously reported as a new group within the genus Bradyrhizobium. To determine their taxonomic position, these strains were characterized in this study using a polyphasic approach. The analysis of the 16S rRNA gene grouped the four strains with Bradyrhizobium pachyrhizi PAC48(T). However, the concatenated sequence analysis of the two (recA and glnII) or three (atpD, gyrB and recA) housekeeping genes indicated that these strains represent a novel species of Bradyrhizobium, which is very closely related to B. pachyrhizi PAC48(T) and B. elkanii USDA 76(T). Genomic relatedness analyses between the UFLA03-321(T) strain and B. elkanii USDA 76(T) and B. pachyrhizi PAC48(T) revealed an average nucleotide identity below 96% and values of estimated DNA-DNA hybridization below 70%, confirming that they represent genomically distinct species. Analysis of MALDI-TOF MS (Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry) profiles and phenotypic characteristics also allowed differentiation of the novel species from its two neighboring species. In phylogenetic analysis of nodC and nifH genes, UFLA03-321(T) exhibited maximum similarity with B. tropiciagri CNPSo 1112(T). The data suggest that these four UFLA strains represent a novel species, for which the name Bradyrhizobium brasilense sp. nov. is proposed, with UFLA03-321(T) (=LMG 29353 =CBAS645) as type strain. G + C content in the DNA of UFLA03-321(T) is 63.9 mol %.
机译：从Vigna Unguiculata的结节（UFLA03-321（T），UFLA03-320和UFLA03-290）和MacroTim Atropurpureum（UFLA04-03-290）和Macropilium Atropurpureum（UFLA04-0212）中的四种菌株以前被报告为Bradylizobium属的新组。为了确定其分类物理位置，这些菌株的特征在于使用多色方法。 16S RRNA基因的分析将四种菌株与Bradyrhizobium pachyrhizi Pac48（t）进行了四种菌株。然而，两个（RECA和GLNII）或三（ATPD，GYRB和RECA）内政基因的连接序列分析表明，这些菌株代表了一种新的BRADYRHIZOBIUM物种，其与B.Pachyrhizi PAC48（T）非常密切相关B. Elkanii USDA 76（T）。 UFLA03-321（T）菌株和B. elkanii USDA 76（T）和B.Pachyrhizi Pac48（t）之间的基因组相关性分析在96％以下的平均核苷酸同一性，估计的DNA-DNA杂交的值低于70％，确认它们代表了基因组不同的物种。 MARDI-TOF MS的分析（基质辅助激光解吸电离 - 飞行时间质谱）曲线和表型特征也允许从其两种相邻物种中分化新种。在NODC和NiFH基因的系统发育分析中，UFLA03-321（T）与B.Tropiciagri CNPSO 1112（T）表现出最大相似性。这些数据表明，这四种UFLA菌株代表了一种新颖的物种，名称Bradyrhizobium Brasilense Sp。 11月。提出，用UFLA03-321（t）（= LMG 29353 = CBAS645）作为型应变。 UFLA03-321（T）的DNA中的G + C含量为63.9mol％。

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