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首页> 外文期刊>Archives of microbiology >Lipid droplets accumulation and other biochemical changes induced in the fungal pathogen Ustilago maydis under nitrogen-starvation
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Lipid droplets accumulation and other biochemical changes induced in the fungal pathogen Ustilago maydis under nitrogen-starvation

机译：在氮饥饿下真菌病原体Ustilago maydis诱导的脂质液滴积累和其他生化变化

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摘要

In many organisms, the growth under nitrogen-deprivation or a poor nitrogen source impacts on the carbon flow distribution and causes accumulation of neutral lipids, which are stored as lipid droplets (LDs). Efforts are in progress to find the mechanism of LDs synthesis and degradation, and new organisms capable of accumulating large amounts of lipids for biotechnological applications. In this context, when Ustilago maydis was cultured in the absence of a nitrogen source, there was a large accumulation of lipid bodies containing mainly triacylglycerols. The most abundant fatty acids in lipid bodies at the stationary phase were palmitic, linoleic, and oleic acids, and they were synthesized de novo by the fatty-acid synthase. In regard to the production of NADPH for the synthesis of fatty acids, the cytosolic NADP(+)-dependent isocitrate dehydrogenase and the glucose-6-phosphate and 6-phosphogluconate dehydrogenases couple showed the highest specific activities, with a lower activity of the malic enzyme. The ATP-citrate lyase activity was not detected in any of the culture conditions, which points to a different mechanism for the transfer of acetyl-CoA into the cytosol. Protein and RNA contents decreased when U. maydis was grown without a nitrogen source. Due to the significant accumulation of triacylglycerols and the particular composition of fatty acids, U. maydis can be considered an alternative model for biotechnological applications.
机译：在许多生物中，氮气剥夺的生长或氮源差的氮源对碳流分布的影响，并导致中性脂质的积累，其被储存为脂液滴（LDS）。努力正在进行中，以找到LDS合成和降解的机制，以及能够积累大量脂质的生物技术应用的新生物。在这种情况下，当在没有氮源的情况下培养ustilago maydis时，含有主要含有三酰基甘油的脂质体积大。固定相的脂质体中最丰富的脂肪酸是棕榈酸，亚油和油酸，它们是通过脂肪酸合成酶合成的Novo。关于NADPH的制备用于合成脂肪酸，胞质NADP（+） - 依赖性异柠檬酸脱氢酶和葡萄糖-6-磷酸酯和6-磷光葡萄糖脱氢酶对夫妇呈现最高的特异性活性，具有较低的麦芽活性酶。在任何培养条件下未检测到ATP-柠檬酸盐酶活性，其指向将乙酰-COA转移到胞浆中的不同机制。当没有氮源时生长蛋白质和RNA含量减小。由于三酰基甘油的显着积累和脂肪酸的特定组合物，U. Maydis可以被认为是生物技术应用的替代模型。

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