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Microvirga rosea sp nov.: a nanoparticle producing bacterium isolated from soil of rose garden

机译:MicroVirga Rosea SP 11月:从玫瑰园土壤中分离的纳米粒子生产细菌

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摘要

A Gram-stain negative, aerobic, non-motile, and rod-shaped novel bacterial strain, designated MAH-2(T), was isolated from a soil sample of rose garden and was characterized using a polyphasic approach. The colonies were light pink color, smooth, circular and 0.2-0.6 mm in diameter when grown on nutrient agar for 3 days. Strain MAH-2(T) grows at 15-40 degrees C (optimum growth temperature 30 degrees C), at pH 5.0-7.0 (optimum growth pH 6.5) and at 0-2% NaCl (optimum 0-0.5%). Cell growth occurs on nutrient agar and R2A agar but not on tryptone soya agar, luria-bertani agar and MacConkey agar. The strain was positive for both catalase and oxidase tests. The strain was able to synthesis of silver nanoparticles. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Microvirga and was most closely related to Microvirga soli R491(T) (96.7% sequence similarity), Microvirga subterranea Fail4(T) (96.4%), Microvirga guangxiensis 25B(T) (96.0%) and Microvirga aerophila 5420S-12(T) (95.9%). The genomic DNA G+C content of isolated strain was determined to be 62.5 mol% and the predominant isoprenoid quinone was Q-10. The major fatty acids were identified as summed feature 8 (comprising C-18:1 omega 7c and/or C-18:1 omega 6c) and C-19:0 cyclo omega 8c. On the basis of these phenotypic, genotypic, and chemotaxonomic studies and DNA-DNA hybridization results, the isolated strain MAH-2(T) represents a novel species, for which the name Microvirga rosea sp. nov. is proposed, with MAH-2(T) as the type strain (=KACC 19290(T)=CGMCC1.16488(T)).
机译:从玫瑰园的土壤样品中分离出革兰污染的阴性,有氧,非运动和棒状新型细菌菌株和指定MAH-2(T),并使用多色方法表征。在营养琼脂上生长3天时,菌落是浅粉红色,光滑,圆形和0.2-0.6mm的直径。菌株MAH-2(T)在pH 5.0-7.0(最佳生长pH6.5)和0-2%NaCl(最佳0-0.5%)下以15-40℃(最佳生长温度30℃)增长。细胞生长发生在营养琼脂和R2A琼脂上,但不存在于胰蛋白酶大豆琼脂,LURIA-BERTANI琼脂和Macconkey琼脂上。菌株对于过氧化氢酶和氧化酶测试是阳性的。该菌株能够合成银纳米颗粒。根据16S rRNA基因序列比较,将分离物鉴定为Microvirga属的成员,与MicroVirga Soli R491(T)最密切相关(96.7%序列相似性),microvirga次果汁缺陷4(t)(96.4%), Microvirga Guangxiensis 25B(T)(96.0%)和MicroVirga Aerophila 5420s-12(t)(95.9%)。将分离的菌株的基因组DNA G + C含量测定为62.5mol%,主要的异戊二烯醌是Q-10。将主要脂肪酸鉴定为总结特征8(包含C-18:1ω7C和/或C-18:1ω6C)和C-19:0环ω8C。在这些表型,基因型和趋化性的研究和DNA-DNA杂交结果的基础上,分离的菌株MAH-2(T)代表了一种新颖的物种,名称MICOVIRGA ROSEA SP。 11月。提出,用MAH-2(t)作为类型应变(= KACC 19290(T)= CGMCC1.16488(T))。

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