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首页> 外文期刊>Archives of Oral Biology >Decreased levels of matrix metalloproteinase-2 in root-canal exudates during root canal treatment
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Decreased levels of matrix metalloproteinase-2 in root-canal exudates during root canal treatment

机译：在根管治疗过程中，在根管中渗出的基质金属蛋白酶-2水平降低

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Highlights ? The presence of proMMP-2 and active MMP-2 in root-canal exudates of patients with pulp necrosis and AAP. ? The absence of proMMP-2 and active MMP-2 in root-canal exudates of patients with normal pulp and periradicular tissues. ? Reduced MMP-2 and MMP-8 levels in root-canal exudates after endodontic treatment in patients with pulp necrosis and AAP. ? The undetectable level of total MMP-2 in root-canal exudates at the last treatment visit. ? Lower MMP-2 and MMP-8 levels in root-canal exudates of negative bacterial cultures than those of positive bacterial cultures. Abstract Objective To determine the matrix metalloproteinase-2 (MMP-2) levels in root-canal exudates from teeth undergoing root-canal treatment. Material and methods The root-canal exudates from six teeth with normal pulp and periradicular tissues that required intentional root canal treatment for prosthodontic reasons and from twelve teeth with pulp necrosis and asymptomatic apical periodontitis (AAP) were sampled with paper points for bacterial culture and aspirated for the detection of proMMP-2 and active MMP-2 by gelatin zymography and the quantification of MMP-2 levels by ELISA. Results By gelatin zymography, both proMMP-2 and active MMP-2 were detected in the first collection of root-canal exudates from teeth with pulp necrosis and AAP, but not from teeth with normal pulp, and their levels gradually decreased and disappeared at the last collection. Consistently, ELISA demonstrated a significant decrease in MMP-2 levels in the root-canal exudates of teeth with pulp necrosis and AAP following root canal procedures ( p 0.05). Furthermore, the MMP-2 levels were significantly lower in the negative bacterial culture than those in the positive bacterial culture ( p 0.001). Conclusions The levels of MMP-2 in root-canal exudates from teeth with pulp necrosis and AAP were gradually reduced during root canal procedures. Future studies are required to determine if MMP-2 levels may be used as a biomolecule for the healing of apical lesions, similar to the clinical application of MMP-8 as a biomarker.
机译：强调 ？ PROMMP-2和活性MMP-2的存在，纸浆坏死和AAP患者的根管渗出物中。还患有正常纸浆和百血体组织的患者的根管渗出物中没有PROMMP-2和活性MMP-2。还在牙髓坏死和AAP患者的牙髓治疗后，在根管治疗后减少MMP-2和MMP-8水平。还在最后一次治疗访问中，根管中的根部总MMP-2的不可检测水平。还降低MMP-2和MMP-8水平的负细菌培养物的根管渗出物，而不是阳性细菌培养物。摘要目的从牙根治疗中确定根管中的基质金属蛋白酶-2（MMP-2）水平。材料和方法用六颗牙齿从六颗牙齿渗出，具有正常的纸浆和百血能组织，需要有意的根管治疗用于假肢的原因，并从12个牙齿与纸浆坏死和无症状的顶端牙周炎（AAP）进行采样，用纸点进行细菌培养并吸入通过明胶酶沉积检测PROMMP-2和活性MMP-2和ELISA的MMP-2水平的定量。通过明胶酶沉思的结果，在从牙髓坏死和AAP的牙齿的第一个根管渗出物中检测到PROMMP-2和活性MMP-2，但不是来自牙髓的牙齿，它们的水平逐渐下降并消失在最后一个集合。始终如一地，ELISA在根管坏死和根部管道中的牙齿坏死和AAP的根管渗出物中显示出MMP-2水平的显着降低（P <0.05）。此外，在阴性细菌培养中的MMP-2水平显着低于阳性细菌培养物（P <0.001）。结论根管手术过程中，用纸浆坏死和AAP渗出牙齿的根管中的MMP-2水平逐渐减少。未来的研究是确定MM​​P-2水平可以用作顶端病变的愈合的生物分子，类似于MMP-8作为生物标志物的临床应用。

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