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The effect of a hydroxyapatite impregnated PCL membrane in rat subcritical calvarial bone defects

机译:羟基磷灰石浸渍PCL膜在大鼠亚临界颅骨骨缺损的作用

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Graphical abstract Display Omitted Highlights ? We created two PCL membranes by electrospinning. ? We compared the effects of the PCL membranes with or without hydroxyapatite in subcritical calvarial bone defects healing in rats. ? Both membranes improved the bone healing. ? Hydroxyapatite improved the PCL membrane bone healing properties. Abstract Objective The present study evaluated the effect of polymeric-nanofibers membranes impregnated with microparticulate hydroxyapatite (HA) in the subcritical calvarial bone defects (SCBD) healing. Design PCL membranes with and without HA were obtained by electrospinning. SCBD were perforated (3.3 mm) in left and right sides of 36 rat calvarias. The right-side SBCD of 18 animals was filled with HA mixed with blood clot and blood clot at the contralateral side. The remaining animals received PCL + HA membrane at the right-side SCBD and PCL membrane at the contralateral side. Animals were killed after 30, 60 and 90 days after surgery. Bone defect volume (in mm 3 ) was measured by tomography (CBCT). Qualitative histological analysis and SBCD area (in mm 2 ) were measured. Quantitative data were submitted to Kruskal-Wallis/Dunn tests. Results Reduction of SBCD volume was observed in all treatments but PCL. Association with HA significantly improved bone healing induced by PCL and blood clot. PCL + HA induced the lowest SBCD volume at 60 and 90 days. Complete bone healing was not observed even at 90 days in SCBD treated with blood clot. In every period, more bone formation was observed for SCBD treated with membranes. Conclusions We concluded that both PCL membrane and HA were able to improve bone healing.
机译:图形抽象显示省略了亮点?我们通过静电纺丝创造了两个PCL膜。还我们比较了PCL膜与或不含羟基磷灰石的副磷灰石在大鼠愈合中的疗效。还两种膜都改善了骨愈合。还羟基磷灰石改善了PCL膜骨愈合特性。摘要目的本研究评估了聚合物 - 纳米纤维膜在亚临界颅骨骨缺损(SCBD)愈合中浸渍有微粒羟基磷灰石(HA)的作用。通过静电纺丝获得具有和不具有HA的PCL膜。 SCBD在36只大鼠Calvarias的左侧和右侧穿孔(3.3mm)。 18只动物的右侧SBCD填充了HA与对侧侧的血凝块和血凝块混合。其余的动物在对侧侧的右侧SCBD和PCL膜处接受PCL + HA膜。手术后30,60和90天后杀死动物。通过断层扫描(CBCT)测量骨缺陷体积(mm 3)。测量定性组织学分析和SBCD区域(mM 2)。定量数据提交给Kruskal-Wallis / Dunn测试。结果在所有治疗中观察到SBCD体积的降低,但PCL。与HA的关系显着改善了PCL和血凝块诱导的骨愈合。 PCL + HA在60和90天诱导最低的SBCD体积。甚至在用血凝块处理的SCBD中的90天内未观察到完全骨愈合。在每个时期,用膜处理的SCBD观察到更多的骨形成。结论我们得出结论,PCL膜和医管局都能够改善骨愈合。

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