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首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >Temperature stability of Poly-[hemoglobin-superoxide dismutase-catalase-carbonic anhydrase] in the form of a solution or in the lyophilized form during storage at-80 degrees C, 4 degrees C, 25 degrees C and 37 degrees C or pasteurization at 70 degrees C
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Temperature stability of Poly-[hemoglobin-superoxide dismutase-catalase-carbonic anhydrase] in the form of a solution or in the lyophilized form during storage at-80 degrees C, 4 degrees C, 25 degrees C and 37 degrees C or pasteurization at 70 degrees C

机译：在溶液或冻干形式的溶液形式的温度稳定性在储存期间 - 80℃，4℃，25℃和37℃或70℃下的冻干形式 C.

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Polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase (Poly-[Hb-SOD-CAT-CA]) contains all three major functions of red blood cells (RBCs) at an enhanced level. It transports oxygen, removes oxygen radicals and transports carbon dioxide. Our previous studies in a 90-min 30mm Hg Mean Arterial Pressure (MAP) sustained hemorrhagic shock rat model shows that it is more effective than blood in the lowering of elevated intracellular pCO(2), recovery of ST-elevation and histology of the heart and intestine. This paper is to analyze the storage and temperature stability. Allowable storage time for RBC is about 1 d at room temperature and 42 d at 4 degrees C. Also, RBC cannot be pasteurized to remove infective agents like HIV and Ebola. PolyHb can be heat sterilized and can be stored for 1 year even at room temperature. However, Poly-[Hb-SOD-CAT-CA] contains both Hb and enzymes and enzymes are particularly sensitive to storage and heat. We thus carried out studies to analyze its storage stability at different temperatures and heat pasteurization stability. Results of storage stability show that lyophilization extends the storage time to 1 year at 4 degrees C and 40d at room temperature (compared to respectively, 42 d and 1 d for RBC). After the freeze-dry process, the enzyme activities of Poly-[SFHb-SOD-CAT-CA] was 100 +/- 2% for CA, 100 +/- 2% for SOD and 93 +/- 3.5% for CAT. After heat pasteurization at 70 degrees C for 2 h, lyophilized Poly-[Hb-SOD-CAT-CA] retained good enzyme activities of CA 97 +/- 4%, SOD 100 +/- 2.5% and CAT 63.8 +/- 4%. More CAT can be added during the crosslinking process to maintain the same enzyme ratio after heat pasteurization. Heat pasteurization is possible only for the lyophilized form of Poly-[Hb-SOD-CAT-CA] and not for the solution. It can be easily reconstituted by dissolving in suitable solutions that continues to have good storage stability though less than that for the lyophilized form. According to the P50 value, Poly-[SFHb-SOD-CAT-CA] retains its oxygen carrying ability before and after long-term storage.
机译：多血红蛋白 - 超氧化物歧化酶 - 碳酶 - 碳酸酐酶（Poly-[Hb-SOD-CAT-CA]）含有在增强水平下红细胞（RBC）的所有三种主要功能。它运输氧气，去除氧自由基并运输二氧化碳。我们以前的90分钟30mm Hg平均动脉压（MAP）持续出血休克大鼠模型的研究表明，它比血液降低的血液更有效（2），恢复心脏的ST升高和组织学和肠道。本文是分析储存和温度稳定性。 RBC的允许储存时间在室温下为约1d，4摄氏度为4℃。此外，RBc不能杀死以去除艾滋病毒和埃博拉等感染剂。 PolyHB可以热灭菌，也可以在室温下储存1年。然而，Poly-[HB-SOD-CAT-CA]含有Hb和酶，酶对储存和热特别敏感。因此，我们进行了研究，以分析其在不同温度和热巴氏杀菌稳定性下的储存稳定性。储存稳定性结果表明，冷冻干燥在室温下在4℃和40D下延伸到1年（与分别为42d和1d，用于RBC）。在冷冻干燥过程之后，CA的Poly-[SFHB-SOD-CAT-CA]的酶活性为100 +/- 2％，对于SOD和93 +/- 3.5％的猫。在70摄氏度下培养2小时后，冻干多[HB-SOD-CAT-CA]保留良好的CA 97 +/- 4％，SOD 100 +/- 2.5％和猫63.8 +/- 4的良好酶活性％。在交联过程中可以添加更多猫以在热杀菌后保持相同的酶比。热巴氏灭菌仅适用于冻干形式的聚 - [Hb-SOD-CAT-CA]，而不是溶液。可以通过溶解在合适的溶液中易于具有良好的储存稳定性的溶解来容易地重构，但是虽然小于冻干形式。根据P50值，Poly-[SFHB-SOD-CAT-CA]在长期储存之前和之后保留其氧携带能力。

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