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首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >A key role of microRNA-26a in the scar formation after glaucoma filtration surgery
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A key role of microRNA-26a in the scar formation after glaucoma filtration surgery

机译：微小RORNA-26A在青光眼过滤手术后瘢痕形成的关键作用

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Glaucoma is one of the leading causes of blind worldwide. Post-operative scar formation of filtering tract was one of the main reasons for failure of glaucoma filtration surgery. In this study, we conducted several experiments to detect the expression of miR-26a in the scar tissue in filtering tract and then detect its potential biological effects as well as its target gene. In our present study, it was found that miR-26a was significantly down-regulated in filtering tract scar. Advanced study on the association between the miR-26a and connective tissue growth factor (CTGF) micro RNA (mRNA) showed that miR-26a was inversely correlated with CTGF mRNA level. Advanced biological studies showed that overexpression of miR-26a could decrease the cell viability and migration ability of human Tenon's fibroblasts (HTFs) fibrosis in vitro model. It was also found that miR-26a might up-regulate the apoptotic level of HTFs. Through protein expression detection and luciferase reporter assay, it was found that miR-26a could produce functions that directly target the CTGF. In conclusion, the key finding of the current study is that miR-26a can suppress the activation of HTFs by transforming growth factor (TGF)- by targeting CTGF. This data indicates that miR-26a plays an essential role in the formation of filtering tract scar and function as a potential drug target.
机译：青光眼是全球失明的主要原因之一。过滤道的术后瘢痕形成是青光眼过滤手术失败的主要原因之一。在这项研究中，我们进行了几个实验，以检测过滤道中瘢痕组织中miR-26a的表达，然后检测其潜在的生物效应以及其靶基因。在我们目前的研究中，发现MiR-26a在过滤道疤痕中显着下调。对miR-26a和结缔组织生长因子（ctgf）微RNA（mRNA）之间的关联的高级研究表明miR-26a与CTGF mRNA水平同时相关。先进的生物学研究表明，MIR-26A的过度表达可以降低人叛徒成纤维细胞（HTFS）纤维化体外模型的细胞活力和迁移能力。还发现MiR-26a可能上调HTF的凋亡水平。通过蛋白质表达检测和荧光素酶报告器测定，发现miR-26a可以产生直接靶向CTGF的功能。总之，目前研究的关键发现是MiR-26a可以通过靶向CTGF来抑制生长因子（TGF）来抑制HTF的激活。该数据表明miR-26a在形成过滤道疤痕和用作潜在药物目标的情况下起着重要作用。

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