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首页> 外文期刊>Archives of Toxicology >Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo
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Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo

机译：口服和体内口服聚苯乙烯微塑性颗粒的摄取和影响

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Evidence exists that humans are exposed to plastic microparticles via diet. Data on intestinal particle uptake and health-related effects resulting from microplastic exposure are scarce. Aim of the study was to analyze the uptake and effects of microplastic particles in human in vitro systems and in rodents in vivo. The gastrointestinal uptake of microplastics was studied in vitro using the human intestinal epithelial cell line Caco-2 and thereof-derived co-cultures mimicking intestinal M-cells and goblet cells. Different sizes of spherical fluorescent polystyrene (PS) particles (1, 4 and 10 mu m) were used to study particle uptake and transport. A 28-days in vivo feeding study was conducted to analyze transport at the intestinal epithelium and oxidative stress response as a potential consequence of microplastic exposure. Male reporter gene mice were treated three times per week by oral gavage with a mixture of 1 mu m (4.55x10(7) particles), 4 mu m (4.55x10(7)particles) and 10 mu m (1.49x10(6) particles) microplastics at a volume of 10mL/kg/bw. Effects of particles on macrophage polarization were investigated using the human cell line THP-1 to detect a possible impact on intestinal immune cells. Altogether, the results of the study demonstrate the cellular uptake of a minor fraction of particles. In vivo data show the absence of histologically detectable lesions and inflammatory responses. The particles did not interfere with the differentiation and activation of the human macrophage model. The present results suggest that oral exposure to PS microplastic particles under the chosen experimental conditions does not pose relevant acute health risks to mammals.
机译：存在通过饮食将人类暴露于塑料微粒的证据。微塑性暴露导致的肠粒子摄取和健康相关效果的数据是稀缺的。该研究的目的是分析体外体外体外体外体外颗粒和体内啮齿动物的微塑性颗粒的摄取和影响。使用人的肠上皮细胞系Caco-2及其衍生的共培养物在体外研究微塑料的胃肠道吸收，其衍生的含有肠M细胞和脚卵细胞。使用不同尺寸的球形荧光聚苯乙烯（PS）颗粒（1,4和10μm）来研究颗粒摄取和运输。进行了在体内进料研究中的28天，分析肠上皮和氧化应激反应的转运作为微塑性暴露的潜在后果。男性报告基因小鼠每周通过口服饲养处理三次，用1μm（4.55×10（7）颗粒），4μm（4.55×10（7）颗粒）和10μm（1.49x10（6）颗粒）MICROPLASTICS以10ml / kg / bw的体积。使用人体细胞系THP-1研究颗粒对巨噬细胞极化的影响，以检测对肠免疫细胞的可能影响。总共，研究结果证明了少数颗粒的细胞吸收。体内数据显示没有组织学检索病变和炎症反应。颗粒不会干扰人巨噬细胞模型的分化和激活。本结果表明，在所选实验条件下对PS微塑性颗粒的口腔暴露不会对哺乳动物产生相关的急性健康风险。

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