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首页> 外文期刊>Archives of Toxicology >Alterations in epididymal proteomics and antioxidant activity of mice exposed to fluoride
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Alterations in epididymal proteomics and antioxidant activity of mice exposed to fluoride

机译：对氟化物暴露于氟化物的反恐蛋白质组学和抗氧化活性的改变

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It is well known that high fluoride results in low fertility. Epididymis is the important place for spermatozoa maturation, which is essential for successful fertilization. In the previous studies, fluoride was reported to damage the epididymal structure of mouse and rabbit. However, the mechanism underlying sodium fluoride (NaF)-induced epididymal toxicity has not yet been well elucidated. The aim of this study is to explore the global protein alterations in epididymis of mice exposed to NaF using the iTRAQ technique. Results showed that 211 proteins were differentially expressed in both 25 and 100 mg/L NaF groups. Some of them have been proved to be important for reproduction, such as low-density lipoprotein receptor-related protein 2 (Lrp2), cytochrome c, testis-specific (Cyct), sorbitol dehydrogenase (Sord), glutathione S-transferases (GSTs), acrosin, beta-defensin 126, cysteine-rich secretory protein (Crisp) 1, and Crisp2. Gene ontology (GO) analysis suggested cellular process, organelle and catalytic activity account for high percent and number of differentially expressed proteins. 171 pathways were found after the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, among which the representative maps, such as ribosome, focal adhesion, and phagosome, were involved. Different functional categories post-translational modification, protein turnover, chaperones; translation, ribosomal structure and biogenesis; cytoskeleton; energy production and conversion are implicated in the Cluster of Orthologous Groups (COG) of proteins analysis. Subsequently, the effect of NaF on the antioxidant activity in epididymis, especially glutathione and glutathione-related enzymes, was evaluated. Results exhibited high fluoride caused low total antioxidant capacity (T-AOC), high methane dicarboxylic aldehyde (MDA), decreased reduced glutathione (GSH), and the glutathione-related enzymes [GSH peroxidase (GPx), GSH reductase (GR), and GSH S-transferase (GST)] changes in activity, protein, and mRNA expressions. In summary, NaF decreased the antioxidant activity of epididymis, especially glutathione and glutathione-related enzymes, as well as iTRAQ results, providing new explanations for the low sperm quality induced by fluoride.
机译：众所周知，高氟化物导致生育率低。附睾是精子成熟的重要场所，这对于成功施肥至关重要。在先前的研究中，据报道氟化物损伤小鼠和兔的附睾结构。然而，氟化钠（NAF）含有的附睾毒性的机制尚未得到很好的阐明。本研究的目的是利用ITRAQ技术探讨暴露于NAF暴露于NAF的小鼠附睾中的全球蛋白质改变。结果表明，211个蛋白质在25和100mg / L NAF组中差异表达。其中一些已被证明对繁殖是重要的，例如低密度脂蛋白受体相关蛋白2（LRP2），细胞色素C，睾丸特异性（CYCT），山梨糖醇脱氢酶（SORD），谷胱甘肽S-转移酶（GSTS） ，亚麻素，β-防御素126，富含半胱氨酸的分泌蛋白（酥）1，和Crisp2。基因本体（GO）分析表明细胞过程，细胞器和催化活性占高分百分比和数量的差异表达蛋白质。在基因和基因组（Kegg）分析的京都百科全书（Kegg）分析之后发现了171个途径，其中涉及代表性地图，例如核糖体，局灶性粘附和吞噬体。不同的功能类别翻译后修饰，蛋白质周转，伴侣;翻译，核糖体结构和生物发生;细胞骨架;能量产生和转化率涉及蛋白质分析的正非群体（COG）簇中。随后，评价NAF对附睾抗氧化活性，尤其是谷胱甘肽和谷胱甘肽相关酶的影响。结果表现出高氟化物引起的总抗氧化能力（T-AOC），高甲烷二羧酸醛（MDA），降低谷胱甘肽（GSH）降低，与谷胱甘肽相关酶[GSH过氧化物酶（GPX），GSH还原酶（GR），以及GSH S转移酶（GST）]活性，蛋白质和mRNA表达的变化。总之，NAF降低了附睾，尤其是谷胱甘肽和谷胱甘肽相关酶的抗氧化活性，以及​​ITRAQ结果，为氟化物诱导的低精子质量提供了新的解释。

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