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首页> 外文期刊>Archives of Toxicology >Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia)
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Combined transcriptomic and proteomic analysis reveals a diversity of venom-related and toxin-like peptides expressed in the mat anemone Zoanthus natalensis (Cnidaria, Hexacorallia)

机译：组合的转录组和蛋白质组学分析揭示了在麦松Zoanthus natalensis（Cnidaria，Hexacorallia）中表达的毒液相关和毒素状肽的多样性

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摘要

Venoms from marine animals have been recognized as a new emerging source of peptide-based therapeutics. Several peptide toxins from sea anemone have been investigated as therapeutic leads or pharmacological tools. Venom complexity should be further highlighted using combined strategies of large-scale sequencing and data analysis which integrated transcriptomics and proteomics to elucidate new proteins or peptides to be compared among species. In this work, transcriptomic and proteomic analyses were combined to identify six groups of expressed peptide toxins in Zoanthus natalensis. These include neurotoxin, hemostatic and hemorrhagic toxin, protease inhibitor, mixed function enzymes, venom auxiliary proteins, allergen peptides, and peptides related to the innate immunity. Molecular docking analysis indicated that one expressed Zoanthus Kunitz-like peptide, ZoaKuz1, could be a voltage-gated potassium channels blocker and, hence, it was selected for functional studies. Functional bioassays revealed that ZoaKuz1 has an intrinsic neuroprotective activity in zebrafish model of Parkinson's disease. Since pharmacological blockade of K-V channels is known to induce neuroprotective effects, ZoaKuz1 holds the potential to be developed in a therapeutic tool to control neural dysfunction by slowing or even halting neurodegeneration mediated by ion-channel hyperactivity.
机译：来自海洋动物的毒液已被认为是一种基于肽的治疗方法的新出现来源。已经研究了来自海洋海葵的几种肽毒素作为治疗铅或药理学工具。应使用大规模测序和数据分析的组合策略进一步强调毒液复杂性，其集成了转录组和蛋白质组学，以阐明在物种中进行比较的新蛋白质或肽。在这项工作中，组合转录组和蛋白质组学分析以鉴定Zoanthus Natalensis中的六组表达肽毒素。这些包括神经毒素，止血和出血性毒素，蛋白酶抑制剂，混合功能酶，毒液辅助蛋白，过敏原肽和与先天免疫有关的肽。分子对接分析表明，一种表达Zoanthus kunitz样肽，Zoakuz1可以是电压门控钾通道阻断，因此选择用于功能性研究。功能性生物测定显示，Zoakuz1在帕金森病的斑马鱼模型中具有内在的神经保护活性。由于已知K-V通道的药理学阻断，因此Zoakuz1保持了在治疗工具中开发的可能性以通过离子通道多动介导的介导的神经变性来控制神经功能障碍。

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