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首页> 外文期刊>Archives of virology >Characterization of Dev-CD-23823 and Dev-CT57, new Autographivirinae bacteriophages infecting Cronobacter spp.
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Characterization of Dev-CD-23823 and Dev-CT57, new Autographivirinae bacteriophages infecting Cronobacter spp.

机译：DEV-CD-23823和DEV-CT57的表征，新的亲卓替氏菌感染Cronobacter SPP。

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Cronobacter spp. are opportunistic pathogenic bacteria responsible for severe infections in neonates. Powdered infant formula has been confirmed to be the source of infection in some cases. Bacteriophages offer a safe means for eliminating this pathogen. In the present study, we characterized two closely related Cronobacter-specific bacteriophages of the proposed genus "GAP227virus". The phages Dev-CD-23823 and Dev-CT57 possessed broad host specificity, as they infected 88% and 80% of the Cronobacter strains tested. Genome sequence comparisons of phages Dev-CD-23823 and Dev-CT57 showed different levels of similarity to the prototype GAP227 phage. The Dev-CT57 phage was highly similar, whereas the Dev-CD-23823 phage showed only 75% sequence identity. A phylogenic tree based on the RNA polymerase (RNAP) gene from selected representatives of the subfamily Autographivirinae confirmed the grouping of Dev-CD-23823, Dev-CT57 and GAP227 in one cluster together with phages PP2, Phi80-18 and PhiR8-01. A common conserved motif was also detected in the RNAP promoters of these phages. The functional activity of these RNAP promoters was confirmed experimentally using a promoter probe vector, and a phage-specific signal was observed; however, some cross-specificity of Dev-CD-23823 and Dev-CT57 promoters was also detected. These results will contribute to our understanding of the biology and evolution of Autographivirinae phages.
机译：Cronobacter SPP。是机会致病细菌，负责新生儿的严重感染。在某些情况下，已确认粉末婴儿配方婴儿配方。噬菌体提供了消除该病原体的安全方法。在本研究中，我们表征了两个密切相关的蜡染杆菌特异性癌症“GAP227Virus”的特异性噬菌体。噬菌体DEV-CD-23823和DEV-CT57具有广泛的宿主特异性，因为它们感染了88％和80％的支架菌株测试。噬菌体DEV-CD-23823和DEV-CT57的基因组序列比较显示出与原型GAP227噬菌体的不同程度的相似性。 DEV-CT57噬菌体非常相似，而DEV-CD-23823噬菌体仅显示了75％的序列同一性。基于亚家族物质的选定代表的基于RNA聚合酶（RNAP）基因的文学发生树证实了DEV-CD-23823，DEV-CT57和GAP227的分组与噬菌体PP2，PP2，PHI80-18和PHIR8-01一起。在这些噬菌体的RNAP启动子中也检测到常见的保守基质。使用启动子探针载体实验证实这些RNAP启动子的功能活性，观察到噬菌体特异性信号;然而，还检测到DEV-CD-23823和DEV-CT57启动子的一些奇异性。这些结果将有助于我们对亲卓申犬噬菌体的生物学和演化的理解。

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