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首页> 外文期刊>Artificial Organs >Comparative Decellularization and Recellularization of Normal Versus Streptozotocin‐Induced Diabetes Mellitus Rat Pancreas
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Comparative Decellularization and Recellularization of Normal Versus Streptozotocin‐Induced Diabetes Mellitus Rat Pancreas

机译：正常与链脲佐菌素诱导的糖尿病大鼠胰腺的比较脱细胞化和透血术

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摘要

Abstract Decellularized (DC) organs/tissues offer a promising scaffold for regenerative bioengineering. However, it is not clear whether the diabetic mellitus (DM) pancreas can be used in decellularized and recellularized bioengineering. For assessment of these questions, murine pancreatic scaffolds of normal, type 1DM (T1DM) and type 2 DM (T2DM) pancreas were generated using a perfusion decellularization technique and assessed by histology, scanning electron microscopy, immunohistochemistry, and enzyme‐linked immunosorbent assay (ELISA). The capacity of DC pancreatic scaffolds to support attachment and proliferation of?human umbilical vein endothelial cells (HUVECs) and MIN‐6 β cells was also assessed. Our results showed that DC pancreatic scaffolds were successfully produced from T1DM and T2DM pancreas and maintained their extracellular matrix (ECM) composition, 3D ultrastructure, and various cytokines. All of the pancreatic scaffolds were sufficiently cytocompatible and were able to support proliferation and adhesion of HUVECs and MIN‐6 β cells. The preliminary results support the biological utility of diabetes mellitus pancreatic scaffolds and pave the way for further investigations to assess the potential ability of using diabetes mellitus pancreas as scaffolds for recellularization and eventual medical applications.
机译：摘要脱细胞（DC）器官/组织为再生生物工程提供了一个有前途的脚手架。然而，目前尚不清楚糖尿病患者（DM）胰腺是否可用于脱细胞化和翻透的生物工程。为了评估这些问题，使用灌注脱细胞化技术产生正常，型1dm（T1dm）和2 dm（t2dm）胰腺的鼠胰支架，并通过组织学，扫描电子显微镜，免疫组织化学和酶联免疫吸附测定评估（ ELISA）。还评估了DC胰支架支持附着和增殖的直流胰腺支架和增殖的能力。还评估了人脐静脉内皮细胞（HUVECS）和MIN-6β细胞。我们的研究结果表明，直流胰腺支架由T1DM和T2DM胰腺成功生产，并保持其细胞外基质（ECM）组成，3D超微结构和各种细胞因子。所有的胰腺支架都足够过于细胞系粘接性，并且能够支持HUVECS和MIN-6β细胞的增殖和粘附。初步结果支持糖尿病胰腺颅骨的生物效用，并铺平了进一步调查的方式，以评估使用糖尿病胰腺作为转速和最终医学应用的支架的潜在能力。

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  • 来源
    《Artificial Organs》 |2019年第4期|共14页
  • 作者

    Huang Ying‐Bao; Mei Jin; Yu Yaling; Ding Yuqiang; Xia Weizhi; Yue Ting; Chen Weijian; Zhou Meng‐Tao; Yang Yun‐Jun;

  • 作者单位

    Department of RadiologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou China;

    Anatomy DepartmentWenzhou Medical UniversityWenzhou China;

    Department of Orthopedic SurgeryShanghai Jiao Tong University Affiliated Sixth People’s;

    Institute of NeuroscienceWenzhou Medical UniversityWenzhou China;

    Department of RadiologyThe Second Affiliated Hospital of Wenzhou Medical UniversityWenzhou China;

    Department of RadiologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou China;

    Department of RadiologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou China;

    Department of SurgeryThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou China;

    Department of RadiologyThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 器官移植术;
  • 关键词

    Decellularization; Diabetes mellitus; Extracellular matrix; Pancreas tissue engineering; Tissue engineering;

    机译：脱细胞化;糖尿病;细胞外基质;胰腺组织工程;组织工程;

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