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Development of xenogeneic decellularized biotubes for off‐the‐shelf applications

机译：开发异种脱细胞生物管，用于现成的应用

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Abstract In earlier studies, we developed in vivo tissue‐engineered, autologous, small‐caliber vascular grafts, called “biotubes,” which withstand systemic blood pressure and exhibit excellent performance as small‐caliber vascular prostheses in animal models. However, biotube preparation takes 4?weeks; therefore, biotubes cannot be applied in emergency situations. Moreover, for responses to various types of surgery, grafts should ideally be readily available in advance. The aim of this study was to develop novel, off‐the‐shelf, small‐caliber vascular grafts by decellularizing in vivo tissue‐engineered xenogeneic tubular materials. Silicone rod molds (diameter: 2?mm, length: 70?mm) placed in subcutaneous pouches of a beagle dog for 4?weeks were harvested with their surrounding connective tissues. Tubular connective tissues were obtained after pulling out the impregnated molds. Subsequently, they were decellularized by perfusion with sodium dodecyl sulfate and Triton X‐100. They were stored as off‐the‐shelf grafts at ?20°C for 1?week. The decellularized grafts derived from the beagle dog were xenogeneically transplanted to the abdominal aortas of rats ( n ?=?3). No signs of abnormal inflammation or immunological problems due to the xenogeneic material were observed. Echocardiography confirmed the patency of the grafts at 1?month after implantation. Histological evaluation revealed that the grafts formed neointima on the luminal surface, and that the graft walls had cell infiltration. Little accumulation of CD68‐positive macrophages in the graft wall was observed. Xenogeneic decellularized tubular tissues functioned as small‐caliber vascular grafts, as well as autologous biotubes. This technology enables the easy fabrication of grafts from xenogeneic animals in advance and their storage for at least a week, satisfying the conditions for off‐the‐shelf grafts.
机译：摘要在早期的研究中，我们在体内组织工程，自体，小口径血管移植物中开发，称为“生物管”，该血压可承受全身性血压，并在动物模型中表现出优异的性能。然而，生物管制剂需要4个？因此，生物管不能应用于紧急情况。此外，对于对各种类型的手术进行响应，应该理想地提前使用移植物。本研究的目的是通过在体内组织工程化的异种管状材料中脱细胞脱细胞，开发新颖的现成的小口径血管移植物。硅树脂棒模（直径：2？mm，长度：70μm）放置在Beagle狗的皮下袋中，用周围的结缔组织收获4个？。在拉出浸渍的模具后获得管状连接组织。随后，通过灌注硫酸钠和Triton X-100灌注脱尖。它们被储存为在搁板上的移植物（在搁板上）1？周。衍生自小猎犬犬的脱细胞移植物被异化移植到大鼠的腹主动脉（n？= 3）。没有观察到由于异种材料引起的异常炎症或免疫问题的迹象。超声心动图在植入后1个月确认了移植物的通畅。组织学评估显示移植物在腔表面上形成了内部，并且移植物壁具有细胞浸润。观察到接枝壁中CD68阳性巨噬细胞的少量积累。异聚脱细胞管状组织作为小口径血管移植物，以及自体生物管。该技术能够容易地从卵烯类动物预先制造从卵烯类动物的移植物，并且它们的储存至少一周，满足搁板移植物的条件。

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