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Ambiguity in the Presentation of Decellularized Tissue Composition: The Need for Standardized Approaches

机译：脱细胞组织成分呈现中的模糊性：需要标准化方法

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摘要

Decellularization offers great potential to the field of tissue engineering, as this method gives rise to scaffold material with the native organ architecture by removing all cellular material and leaving much of the extracellular matrix (ECM) intact. However, many parameters may affect decellularization efficacy and ECM retention and, therefore, decellularization protocols need to be optimized for specific needs. This requires robust methods for comparison of decellularized tissue composition. Various representation methods are used in literature to express tissue composition (DNA, glycosaminoglycans, collagen, other ECM proteins, and growth factors). Here, we present and compare the various methods used and demonstrate that normalization to either dry or wet decellularized weight might be misleading and may overestimate true component retention. Moreover, the magnitude of the confounding effect is likely to be decellularization treatment dependent. As a result, we propose alternative comparison strategies: normalization to whole organ or to a unit of whole initial organ weight. We believe proper assessment of decellularized tissue composition is paramount for the successful comparison of different decellularization protocols and clinical translation.
机译：脱细胞化为组织工程领域提供了很大的潜力，因为该方法通过除去所有细胞材料并留下大部分细胞外基质（ECM）完好无损地引起天然器官架构的支架材料。然而，许多参数可能影响脱细胞化功效和ECM保留，因此需要针对特定​​需求进行优化脱细胞化方案。这需要稳健的方法，用于比较脱细胞化组织组成。各种表示方法用于文献中以表达组织组合物（DNA，糖胺聚糖，胶原，其他ECM蛋白和生长因子）。在这里，我们展示并比较了所用的各种方法，并证明了对干燥或湿脱细胞的重量的标准化可能是误导性的，并且可能估量真正的成分保持。此外，混杂效果的大小可能是依赖性的脱细胞化处理。因此，我们提出了替代的比较策略：整个器官的归一化或整个初始器官重量的单位。我们认为对脱细胞组织成分的适当评估对于不同脱细胞化方案和临床翻译的成功比较至关重要。

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