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首页> 外文期刊>Australasian Plant Pathology >DArTseq molecular markers for resistance to Phytophthora cinnamomi in pineapple (Ananas comosus L.)
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DArTseq molecular markers for resistance to Phytophthora cinnamomi in pineapple (Ananas comosus L.)

机译：Dartseq分子标记用于菠萝（Ananas Comosus L.）抗植物抗植物肺炎肉瘤

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摘要

Root rot caused by Phytophthora cinnamomi is a major disease of pineapple worldwide, and while genetic resistance is known to exist within the genus, there have been few efforts to incorporate resistance into commercial varieties. The development of molecular markers and a better understanding of the physiology contributing to resistance would be a major advantage for breeding efforts. DArTseq was used in this study to investigate resistance to P. cinnamomi in pineapple. Resistance appears closely associated with a single broad loci on chromosome five covering the positions 4.6-5.5 Mb and two separate scaffolds. Thirteen putative resistance/susceptibility proteins were identified including six leucine-rich repeat receptor kinases, a chitin elicitor receptor kinase, a 2, 3-bisphosphoglycerate-dependent phosphoglycerate mutase, a microrchidia 4-like protein, a hypothetical protein, an uncharacterised protein family 0664 stress-induced protein, a transcription factor bHLH and a ribonuclease 2. The DArTseq bulk segregate approach successfully identified molecular markers associated with resistance to P. cinnamomi in pineapple.
机译：由植物植物引起的根腐肉瘤是全世界菠萝的主要疾病，而众所周知，遗传性抵抗在属内存在，则几乎没有努力将抗性掺入商业品种。分子标记的发展和更好地理解有助于抵抗的生理学将是育种努力的主要优势。在本研究中使用Dartseq在菠萝中调查抗P. Cinnamomi的抵抗力。阻力与染色体五个覆盖物的染色体覆盖物的单个宽松基因座密切相关。鉴定了13个推定的抗性/易感性蛋白，包括六个富氨氨酸的重复受体激酶，丁蛋白Elicitor受体激酶，a 2,3-双磷酸酯依赖性磷酸甘油蛋白酶蛋白酶，MicroRchidia 4样蛋白，假设蛋白质，一个无特征化的蛋白质家庭0664应激诱导的蛋白质，转录因子BHLH和核糖核酸酶2. DARTSEQ散装分离方法成功鉴定了菠萝中对肉桂瘤的抗性相关的分子标记。

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