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Single-Molecule Studies in Live Cells

机译:活细胞中的单分子研究

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Live-cell single-molecule experiments are now widely used to study complex biological processes such as signal transduction, self-assembly, active trafficking, and gene regulation. These experiments' increased popularity results in part from rapid methodological developments that have significantly lowered the technical barriers to performing them. Another important advance is the development of novel statistical algorithms, which, by modeling the stochastic behaviors of single molecules, can be used to extract systemic parameters describing the in vivo biochemistry or super-resolution localization of biological molecules within their physiological environment. This review discusses recent advances in experimental and computational strategies for live-cell single-molecule studies, as well as a selected subset of biological studies that have utilized these new technologies.
机译:Live-Cell单分子实验现在广泛用于研究复杂的生物过程,如信号转导,自组装,活性贩运和基因调控。 这些实验增加了普及的普及,部分地从快速的方法发展中显着降低了执行它们的技术壁垒。 另一个重要提前是开发新的统计算法,其通过对单一分子的随机行为进行建模,可用于提取描述其生理环境内生物学分子的体内生物化学或超分辨率定位的全身参数。 该综述讨论了最近的实验和计算策略对活细胞单分子研究的进展,以及利用这些新技术的选定生物学研究子集。

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