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In Vitro Multiplication of Cleopatra mandarin from Nodal Explants

机译:克斯科特拉普通话从节点外植体的体外繁殖

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The present investigation was undertaken to develop in vitro protocol for production of Cleopatra mandarin. Nodal segments cultured on Murashige and Skoog medium supplemented with BAP 0.5 mg I~(-1)+ Kin 0.5 mg I~(-1) resulted in earliest shoot initiation and maximum number of shoots per ex-plant. Maximum shoot length was in MS media fortified with BAP 1.0 mg I~(-1)and 0.5 mg I'1 Kin. Rooting media comprising of MS media fortified with IBA0.5 mgl~(-1)or IBA1.0 mg I~(-1) resulted in 100% rooting of micro shoots. Full strength MS medium with IBA 1.0 mg/l required minimum days for root initiation. Half strength MS media supplemented with IBA2.0 mg I~(-1) and IBA1.0 mg I~(-1) resulted in maximum average number of roots per micro shoot and maximum root length respectively. The highest survival per cent (90%) of in vitro grown plants was in potting media containing sand, soil and vermi compost in 1:1:1 ratio.
机译:本发明的调查是为生产克利奥帕特拉普通话的体外方案开发。 在Murashige和Skoog培养基上培养的节点段,其补充有BAP 0.5mg I〜(-1)+ kin 0.5mg I〜(-1)导致每种前植物的最早拍摄和最大芽数。 最大射击长度在MS介质中强化BAP 1.0 mg I〜(-1)和0.5 mg'1 kin。 生根介质,包括用IBA0.5 MGL〜(-1)或IBA1.0mg I〜(-1)强化的MS培养基导致100%的微芽生根。 具有IBA 1.0 mg / L的全强度MS培养基需要根起始最短的天数。 补充有IBA2.0mg I〜(-1)和IBA1.0mg I〜(-1)的半强度MS培养基导致每微小芽和最大根长度的最大平均根数。 体外生长植物的最高生存率(90%)在1:1:1的比例中含有砂,土壤和绒毛堆肥的灌封介质。

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