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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Quercetin and silver nitrate modulate organogenesis in Carissa carandas (L.)
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Quercetin and silver nitrate modulate organogenesis in Carissa carandas (L.)

机译:槲皮素和硝酸银在Carissa Carandas(L.)中调节组织组织

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摘要

An in vitro organogenesis protocol for Carissa carandas L. was developed using an auxin transport inhibitor (quercetin) and silver nitrate (AgNO3), an inhibitor of ethylene action, in association with cytokinins in the culture medium. This protocol produced the maximum number of shoots from aseptic seedling-derived shoot apex explants of C. carandas. The highest rate of shoot multiplication was recorded on MS medium containing 2.0 mg L-1 6-benzylaminopurine; 0.5 mg L-1 kinetin, and 0.75 mg L-1 quercetin at after 4 wk of culture. Similar results were obtained when MS medium fortified with 2.0 mg L-1 BAP, 0.5 mg L-1 kinetin, and 1.5 mg L-1 AgNO3 was used. However, successful rooting was achieved on quarter strength MS medium with 0.5 mg L-1 indole-3-acetic acid. In this study, an inhibitor of auxin transport and ethylene action maximized shoot multiplication in medium fortified with cytokinins. The established rapid micropropagation method could be used to conserve elite genotypes of C. carandas.
机译:使用植物素转运抑制剂(槲皮素)和硝酸银(AgNO3),乙烯作用抑制剂,与培养基中的细胞素相关,乙烯作用抑制剂(AgNO3)的体外有机组合方案。该方案产生了来自C. Carandas的无菌幼苗源芽外植体的最大芽数。在含有2.0mg L-1 6-苄基氨基嘌呤的MS培养基上记录出最高次次升量速率; 0.5mg L-1 Kinetin,4周后的0.75mg L-1槲皮素在4周后。当使用2.0mg L-1 BAP强化0.5mg L-1 kinetin和1.5mg L-1 AgNO 3时,获得了类似的结果。然而,在四分之一强度MS培养基上实现成功的生根,其中0.5mg L-1吲哚-3-乙酸。在该研究中,毒素转运抑制剂和乙烯作用最大化的血液中的血液升压,含有细胞分裂素。建立的快速微磁化方法可用于保护C. Carandas的Elite基因型。

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