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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Efficient callus-mediated regeneration and in vitro root tuberization in Trichosanthes kirilowii Maxim., a medicinal plant
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Efficient callus-mediated regeneration and in vitro root tuberization in Trichosanthes kirilowii Maxim., a medicinal plant

机译：高效的愈伤组织介导的再生和Trichosanthes kirilowii maxim中的体外根核化。，药用植物

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Trichosanthes kirilowii Maxim. is a climbing herb with considerable medicinal value. In this study, efficient protocols for callus-mediated regeneration and in vitro tuberization of this plant were developed. Sterilized stem and leaf tissues were cultured on Murashige and Skoog (MS) medium with plant growth regulators (PGRs), and additives that promoted callus induction and regeneration. Both stem and leaf tissues showed the best response (100%) for callus initiation on MS medium supplemented with 4.5-mu M 2,4-dichlorophenoxyacetic acid (2,4-D). Efficient shoot organogenesis was obtained by exposing the callus tissue to 4.6-mu M kinetin, 2.2-mu M 6-benzylaminopurine, and 2.7-mu M 1-naphthylacetic acid (NAA) along with 12.6-mu M copper sulfate, which yielded a shoot regeneration rate of 85.5% and 28 shoots derived from each callus. In vitro shoots were best rooted on half-strength (1/2) MS medium with 2.7-mu M NAA. Tuberous roots were efficiently induced on rooting medium with 5% (w/v) sucrose under short illumination conditions (8 h photoperiod). Rooted plantlets were successfully acclimatized in pots with a & 90% survival rate. This protocol provides an effective method for callus-mediated regeneration and in vitro root tuberization.
机译：Trichosanthes Kirilowi​​i Maxim。是一个攀岩草药，具有相当大的药用价值。在该研究中，开发了愈伤组织介导的再生和该植物的体外结核的有效方案。灭菌的茎和叶组织在Murashige和Skoog（MS）培养基上用植物生长调节剂（PGR）和促进愈伤组织诱导和再生的添加剂。茎和叶组织均显示出补充有4.5-mu m 2,4-二氯苯乙酸（2,4-D）的MS培养基上的愈伤组织发酵的最佳反应（100％）。通过将愈伤组织组织暴露于4.6-mu m kinetin，2.2-mu m 6-苄基氨基嘌呤和2.7-mu m 1-萘酰基乙酸（Naa）以及12.6-mu m铜硫酸盐来获得有效的射肌器官发生。产生芽再生率为85.5％，28个芽源自每个愈伤组织。体外芽最适合于含有2.7亩NAA的半强度（1/2）MS培养基。在短照明条件下用5％（w / v）蔗糖的生根培养基有效地诱导结节根（8小时光周期）。生根的小植株在具有A＆amp的盆中成功地置身于盆中; GT; 90％存活率。该方案提供了愈伤组织介导的再生和体外根核化的有效方法。

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