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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Micropropagation of virus-free plants of Saudi fig (Ficus carica L.) and their identification through enzyme-linked immunosorbent assay methods
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Micropropagation of virus-free plants of Saudi fig (Ficus carica L.) and their identification through enzyme-linked immunosorbent assay methods

机译：通过酶联免疫吸附测定方法的Saudi（Ficus Carica L.）的无病毒植物的微催化及其鉴定

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摘要

Viral infection is one of the most serious biotic stresses, which disturbs the growth and productivity of many horticultural crops, including that of fig (Ficus carica L.). The production of plants free of viruses, such as fig mosaic virus (FMV), has become a priority in many plant breeding programs. In this study, leaves from plants of two fig cultivars, Kodato and Dattora, infected with FMV were collected from both Mecca and Al-Taif, Saudi Arabia. Transmission electron microscopy of ultrathin leaf sections showed double membrane bodies, characteristic of FMV particles, only in the mesophyll cells of infected samples. Protein analysis using sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of a protein band with a molecular weight of 35 kDa, which corresponded to the viral coat protein; and FMV was confirmed by Western blot and enzyme-linked immunosorbent assay (ELISA) tests. To obtain virus-free plants, apical shoot culture was applied. A comparison of various artificial media with different concentrations of growth regulators was evaluated to optimize shoot formation, shoot multiplication, and root formation, and was followed by plant acclimation ex vitro. Direct ELISA analysis of shoots micropropagated from meristem tip explants indicated that there were virus-free shoots, when compared to infected plants (positive control), while there were no significant differences between these explants and healthy samples (negative control). This study demonstrated that in vitro micropropagation of Saudi F. carica infected with FMV virus led to the successful elimination of the virus.
机译：病毒感染是最严重的生物应力之一，它扰乱了许多园艺作物的生长和生产力，包括图（Ficus Carica L.）的生长和生产率。没有病毒的植物的生产，例如无花果病毒（FMV），已成为许多植物育种计划中的优先事项。在这项研究中，从沙特阿拉伯的麦加和Al-Taif都收集了来自两个无花果，kodato和dattora的植物的叶子。超薄叶片部分的透射电子显微镜显示出双膜体，仅在感染样品的叶片细胞中的FMV颗粒的特征。蛋白质分析使用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳显示出蛋白质带的存在，其中分子量为35kDa，其对应于病毒外壳蛋白质;通过蛋白质印迹和酶联免疫吸附测定（ELISA）测试证实了FMV。为了获得无病毒植物，施加顶端芽培养。评价各种人工介质具有不同浓度的生长调节剂的比较，以优化芽形成，射击倍增和根部形成，然后进行植物适应exturo。直接ELISA分析枝条微催化从Meristem Tip外植体表明，与受感染的植物（阳性对照）相比，无病毒芽，而这些外植体和健康样品（阴性对照）之间没有显着差异。本研究表明，Saudi F. Carica的体外微扑发感染FMV病毒导致了病毒的成功消除。

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