...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >An efficient protocol for in vitro regeneration and conservation of Shirui lily (Lilium mackliniae Sealy): a lab-to-land approach to save the rare endangered Asiatic lily species
【24h】

An efficient protocol for in vitro regeneration and conservation of Shirui lily (Lilium mackliniae Sealy): a lab-to-land approach to save the rare endangered Asiatic lily species

机译：一种高效的体外再生和保护Shirui Lily（Lilium Mackliniae Sealy）的培养方案：拯救罕见濒危亚洲百合物种的实验室对陆地方法

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An efficient protocol for direct and indirect shoot regeneration and proliferation from bulb scales of Shirui lily (Lilium mackliniae Sealy), an endangered Asiatic lily species endemic to the Shirui hill peak, Manipur, India, has been developed. Bulb scales were isolated from mature bulbs and cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 6-benzylaminopurine (BAP), kinetin (KIN), or thidiazuron (TDZ). For direct shoot regeneration from bulb scale explants, 0.5 mg L-1 BAP yielded the highest shoot induction (3.5 shoots per scale; a 96.7% response). For indirect de novo organogenesis, optimum callus induction was achieved with 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), and shoot organogenesis was higher (16.2) when subcultured onto 0.5 mg L-1 BAP medium. Multiple shoot regeneration and pseudo-bulb formation protocols were assessed; the highest shoot proliferation (10.1) occurred with 0.5 mg L-1 BAP and 1.0 mg L-1 gibberellic acid (GA(3)). Rooting response was 96% with 0.5 mg L-1 1-naphthalene acetic acid (NAA), with multiple roots per shootlet. Plantlet survival was increased to 92.5% during the hardening-off process by using hydroponics with Hoagland's solution in a mist chamber. Clonal fidelity was assessed through random amplified polymorphic DNA (RAPD) analysis comparing the mother plant and regenerated plantlets. After confirming genetic uniformity, the pseudo-bulblets with four to six leaves and three to four roots were successfully established at the Shirui hills peak. This in vitro regeneration and ex vitro conservation approach could be helpful to save this rare endangered species in a sustainable way.
机译：已经开发出一种高效的直接和间接射击再生和来自Shiru Lily（Lilium Mackliniae Seawy）的灯泡级别，这是一种濒危亚洲百合物种，这是一个濒危亚洲百合物种的印度，印度的Shirui Hill Peak，Indipur，Indipur。从成熟灯泡中分离出灯泡鳞片，并在Murashige和Skoog（MS）基础培养物上培养，其补充有不同浓度的6-苄氨基嘌呤（BAP），Kinetin（Kin）或Thidiazuron（TDZ）。对于灯泡尺度外植体的直接拍摄再生，0.5mg L-1 BAP产生最高的芽诱导（每尺3.5次枝条; 96.7％的反应）。对于间接De Novo的有机组织，用2.0mg L-1 2,4-二氯苯氧基乙酸（2,4-D）实现最佳愈伤组织诱导，并且当转培养到0.5mg L-1 Bap培养基上时，芽组织较高（16.2）。评估多次芽再生和伪灯泡形成方案;最高的芽增殖（10.1）发生，0.5mg L-1 BAP和1.0mg L-1甘油酸（Ga（3））。生根响应为96％，0.5mg L-1 1-萘乙酸（NAA），每次射击多个根。通过在雾气室中使用水栽法在硬化过程中，植物存活率增加到92.5％。通过随机扩增的多晶态DNA（RAPD）分析评估克隆待遇，比较母植物和再生植物。在确认遗传均匀性之后，在Shirui Hills Peak上成功建立了有4到六片叶子和三到四根叶片的伪推子。这种体外再生和前体外保存方法可能有助于以可持续的方式节省这种罕见的濒危物种。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号