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首页> 外文期刊>In Vitro Cellular and Developmental Biology. Animal: Journal of the Tissues Culture Association >Influence of storage time and nutrient medium on recovery of fibroblast-like cells from refrigerated collared peccary (Pecari tajacu Linnaeus, 1758) skin
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Influence of storage time and nutrient medium on recovery of fibroblast-like cells from refrigerated collared peccary (Pecari tajacu Linnaeus, 1758) skin

机译：储存时间和营养培养基对冷藏套牙科（Pecari Tajacu Linnaeus，1758）皮肤恢复成纤维细胞样细胞的影响

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摘要

Animal cloning is a promising technology for biodiversity conservation, and its success depends on the recovery of nucleus donor cells. Specifically for collared peccaries, found sometimes in regions that are difficult to access, the storage at 4-6 degrees C of skin tissues would be an alternative for the conservation of genetic material. Therefore, we aimed to evaluate different storage periods and the presence of a nutrient medium at 4-6 degrees C on the recovery of somatic cells from the skin of collared peccaries. To analyze cell recovery rates, ear explants were distributed in non-refrigerated samples and samples refrigerated for 10, 30, and 50 d in the absence or presence of nutrient medium. All explants were analyzed by histologically and cultured. Only the fragments stored for 50 d without medium showed an increase in the total thickness of skin. Moreover, increased storage period, regardless of the presence of medium, increased the halo number and reduced the metabolic activity. After culture, only the fragments stored without medium for 50 d did not yield any somatic cells. Cells recovered from explants stored for 10 d showed similar characteristics to these recovered from non-refrigerated explants, regardless of the presence of medium, including the day at which explants achieved attachment and the total time to reach subconfluence. In conclusion, viable cells can be recovered from somatic tissues of collared peccaries stored for up to 50 d in the presence of medium, and tissues refrigerated for up to 10 din the presence of medium yielded more viable cells.
机译：动物克隆是一种有希望的生物多样性保护技术，其成功取决于核供体细胞的复苏。专门针对颈体的剖宫产，有时在难以访问的区域中，皮肤组织的4-6摄氏度的储存是遗传材料的替代方案。因此，我们的目标是在4-6℃下评估不同的储存周期和营养培养基的存在，从锁骨的丘脑皮肤的皮肤中恢复体细胞。为了分析细胞回收率，在不存在或存在营养培养基的情况下，在非冷藏样品中分布在非冷藏样品和样品中的样品和50d的样品。通过组织学和培养分析所有外植体。仅储存50d没有培养基的片段显示皮肤总厚度的增加。此外，不管培养基的存在，增加储存时期，增加了卤素数并降低了代谢活性。培养后，仅储存的片段没有培养基50d没有产生任何体细胞。从储存10 d的外植体回收的细胞显示出与非冷藏外植体回收的类似特征，无论培养基的存在如何，包括介质的存在植物所取得的附着的那一天和达到分组的总时间。总之，在介质存在下，可以从储存最多50d的环形丘脑的体细胞中回收活细胞，并且冷藏最多10丁种的组织，培养基的存在产生了更活力的细胞。

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