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RAPD PROFILING OF OXYOPIDAE SPIDERS

机译:oxoxopidae蜘蛛的RAPD剖析

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摘要

Random amplified polymorphic DNA(RAPD) Polymerase Chain Reaction (PCR) was used to characterize six spiders belonging to the family Oxyopidae. The aim was to achieve genetic marker to determine the genetic variations of these species. The species studied includes: Oxyopes lineatipes (C L Koch, 1847), O. javanus (Thorell, 1887), O. shweta (Tikader, 1970), O. sunandae (Tikader, 1970), Hamadruas sp. (Thorell, 1887) and Peucetia viridana (Stolickza, 1869) collected from Karnataka. RAPD-PCR was carried out by using three random primers which generated polymorphic fragments. Genetic polymorphism among these samples was recorded by Neighbour Joining (NJ) method. The results revealed that their genetic distance ranged from 0.6962- 0.8523. Cluster analysis based on this revealed that the tree is divided into two clades. The results concluded that the RAPD-PCR is an effective, fast and simple alternative method for identifying these spiders.
机译:随机扩增的多晶态DNA(RAPD)聚合酶链反应(PCR)用于表征属于oxoxopidae的六个蜘蛛。 目的是达到遗传标记,以确定这些物种的遗传变化。 所研究的物种包括:氧气opes lineatipes(c l Koch,1847),O. javanus(Thorell,1887),O. Shweta(Tikader,1970),O. Sunandae(Tikader,1970),哈马德鲁斯SP。 (Thorell,1887年)和Peucetia Viridana(Stolickza,1869)从卡纳塔克邦收集。 通过使用产生多态性片段的三种随机引物进行RAPD-PCR。 通过邻居连接(NJ)方法记录这些样品中的遗传多态性。 结果表明,它们的遗传距离范围为0.6962- 0.8523。 基于此的聚类分析显示,树分为两种分隔。 结果得出结论,RAPD-PCR是一种用于识别这些蜘蛛的有效,快速而简单的替代方法。

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