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首页> 外文期刊>Indian Journal of Fisheries >Isoaltion and characterisation of Vibrio harveyi as etiological agent of foot pustule disease in the abalone Haliotis discus hannai Ino 1953
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Isoaltion and characterisation of Vibrio harveyi as etiological agent of foot pustule disease in the abalone Haliotis discus hannai Ino 1953

机译：Abalone Haliotis Discus Hannai Ino中鲍氏脓疱病病因患者的vibrio harveyi的分离与表征1953

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摘要

Foot pustule disease is an important disease affecting abalones. In the present study, microbiological and histopathological investigations were carried out in the Japanese abalone Haliotis discus hannai, affected by foot pustule disease. Diseased abalones became lethargic, weak, and eventually died. The remarkable symptoms were apparent foot pustules and atrophy of the foot muscle. A predominant bacterium designated as BV2 was isolated from the pustules of diseased abalones. Experimental infection by immersion challenge showed that BV2 was virulent to abalones and caused symptoms of foot pustule disease with median lethal dose value of approximate to 7.76x10(5) cfu ml(-1). BV2 was infectious to all tested abalones with high mortality. Histopathological investigations showed degeneration and collapse of foot muscles as well as connective tissues. Tissues in round pustules were necrotic and disorganised. The BV2 bacterium was identified as Vibrio harveyi based on the results of phenotypic and biochemical tests as well as 16S rRNA gene sequence analysis.. The bacterium was found resistant to kanamycin and clindamycin, and sensitive to other 11 antibiotics tested.
机译：脚脓病是一种影响鲍鱼的重要疾病。在目前的研究中，在日本鲍鱼Haliotis Discus hannai中进行微生物和组织病理学调查，受足脓病症的影响。患病的鲍鱼变得昏昏欲睡，弱，最终死亡。显着的症状是表观脚脓肿和脚肌的萎缩。从患病鲍鱼的脓液中分离出作为BV2被指定的主要细菌。浸没挑战的实验感染表明，BV2是毒性的紫罗兰，并导致患者致命剂量值的患者致命剂量值近似为7.76×10（5）CFU mL（-1）。 Bv2对所有受到高死亡率的鲍鱼感染了感染性。组织病理学调查显示出脚肌的退化和塌陷以及结缔组织。圆形脓疱中的组织是坏死和杂乱的。基于表型和生化试验的结果以及16S rRNA基因序列分析，将BV2细菌鉴定为vibrio harveyi ..发现细菌对卡那霉素和克林霉素耐药，并对其他11种抗生素敏感。

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