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首页> 外文期刊>Indian Journal of Small Ruminants >MOLECULAR DETECTION OF THEILERIA LUWENSHUNI IN SHEEP AND GOATS OFCHENNAI, TAMIL NADU
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MOLECULAR DETECTION OF THEILERIA LUWENSHUNI IN SHEEP AND GOATS OFCHENNAI, TAMIL NADU

机译：泰米尔纳奈山羊羊和山羊山羊的分子检测

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摘要

Theileriosis is an important haemoprotozoan disease of sheep and goats in tropical and subtropical regions caused by the different species of Theileria (Altay et al., 2007). It is mainly transmitted trans-staidly by various tick vectors of family Ixodidae and cause heavy economic losses (US$ 62 million) to livestock (Li et al., 2014). T. lestoquardi is the most important and highly pathogenic species causing malignant theileriosis in sheep (Friedhoff, 1997). In addition, two newly reported species (T. luwenshuni and T. uilenbergi) from China are also pathogenic to small ruminants (Ahmed et al., 2006; Yin et al., 2007). The other species like T. ovis, T. separata and T. recondita are non-pathogenic in small ruminants (Altay et al., 2007; Alessandra and Santo, 2012). Generally, the diagnosis of small ruminant piroplasmosis is based on microscopic examination of blood and lymph node smears and clinical symptoms. These methods are reliable in acute cases, but of limited value in chronic cases. Similarly, serological techniques, although widely used, have limitations of cross-reactivity, low sensitivity and specificity. Polymerase chain reaction (PCR) based detection of Theileria-specific genes has been reported to determine Theileria infections with high sensitivity and specificity (Altay etal., 2005; HeidarpourBami etal., 2009). Further restriction fragment length polymorphism (RFLP) assay enables differentiation of Theileria spp. Thus, a study was carried out to detect the Theileria spp. in sheep and goats reared in and around Chennai, Tamil Nadu using nested PCR-RFLP.
机译：Theileriosis是由不同种类的不同种类的热带和亚热带地区的绵羊和山羊的重要血红蛋作神（Altay等，2007）。它主要由Ixodidae的各种蜱型载体转过来传播，并将经济损失重大（6200万美元）到牲畜（Li等，2014）。 T. Lestoquardi是羊群中最重要和最致病的物种（Friedhoff，1997）。此外，来自中国的两种新报告的物种（T.Luwenshuni和T.uilenbergi）也是小反刍动物的病原（Ahmed等，2006; Yin等人，2007）。其他物种如T.Ovis，T. seconda和T. Regondita在小型反刍动物中是非致病性（Altay等，2007; Alessandra和Santo，2012）。通常，小反刍动物粒子菌病的诊断是基于血液和淋巴结涂片的微观检查和临床症状。这些方法在急性病例中可靠，但在慢性案件中有限。类似地，血清技术虽然广泛使用，但具有交叉反应性，低灵敏度和特异性的局限性。据报道，基于聚合酶链反应（PCR）的特异性基因的检测，以确定具有高敏感性和特异性的细胞感染（Altay Etal，2005; Heidarpourbami Eetal。，2009）。进一步的限制性片段长度多态性（RFLP）测定能够分化Theileria SPP。因此，进行了一种研究以检测Theileria SPP。在Chennai的绵羊和山羊，泰米尔纳德邦使用嵌套的PCR-RFLP。

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