...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Inorganic Chemistry Frontiers >ESI-TOF MS analysis and DNA cleavage activity of rutin-metal complexes in aqueous extracts of medicinal plants
【24h】

ESI-TOF MS analysis and DNA cleavage activity of rutin-metal complexes in aqueous extracts of medicinal plants

机译：芦丁 - 金属配合物在药用植物水提取物中的ESI-TOF MS分析和DNA切割活性

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rutin is a widely used natural antioxidant and possesses metal ion chelation properties. We used high performance liquid chromatography (HPLC) to analyze rutin in samples from four medicinal plants, obtained from the dissolution of aqueous extracts in two different solvents. The results showed that the peak areas for rutin in the samples dissolved in 15% formic acid-methanol were significantly larger than those from the samples dissolved in methanol alone, with increases in the peak area being 222.68% (Sophorae Flos), 125.50% (Lonicerae Japonicae Flos), 24.81% (Forsythiae Fructus), and 200.47% (Violae Herba). Therefore, HPLC was used to measure the changes in the rutin content before and after the reactions of rutin with eight common trace metal elements (calcium, magnesium, zinc, iron, copper, nickel, manganese, and strontium) in hot water. The results showed that the ability of rutin to form complexes was the strongest with iron, followed by strontium and copper. In addition, electrospray ionization-time of flight mass spectrometry analysis performed on rutin-metal ion reaction supernatants revealed that rutin could form complexes at different ratios with the eight different types of metal ions. Measurements of DNA cleavage activity showed that the rutin-copper complex led to dose- and time-dependent DNA cleavage through oxidative damage, which was not observed when rutin alone was reacted with DNA. The aforementioned results indicate that the organic constituents of plants form complexes with trace metal elements during the water decoction process, contributing to their health promoting and disease treatment effects.
机译：芦丁是一种广泛使用的天然抗氧化剂，具有金属离子螯合性能。我们使用高效液相色谱（HPLC）来分析来自四种药用植物的样品中的芦丁，从两种不同溶剂中的水萃取物溶解。结果表明，溶解在15％甲酸 - 甲醇中的样品中芦丁的峰面积显着大于单独溶解在甲醇中的样品的样品，峰面积增加222.68％（Sophorae Flos），125.50％（汉纶粳稻葡萄酒，24.81％（连翘果酱），200.47％（Violae Herba）。因此，HPLC用于测量芦丁含量的芦丁含量的变化，在芦丁与八个常见的痕量金属元素（钙，镁，铁，铜，镍，锰和锶）在热水中的反应。结果表明，芦丁形成复合物的能力是熨斗中最强的，其次是锶和铜。此外，对芦丁 - 金属离子反应上清液进行的飞行量谱分析的电喷雾电离 - 透露，芦丁可以在不同比例与八种不同类型的金属离子以不同比例形成复合物。 DNA切割活性的测量表明，芦丁 - 铜复合物通过氧化损伤导致剂量和时间依赖性DNA切割，当单独的芦丁与DNA反应时未观察到这一点。上述结果表明，在水煎剂过程中，植物的有机成分形成痕量金属元素，有助于其健康促进和疾病治疗效果。

著录项

  • 来源
    《Inorganic Chemistry Frontiers》 |2019年第11期|共12页
  • 作者

    Cai Weilan; Zheng Kangkang; Li Zhimin; Peng Liang; Yin Qianrong; Zeng Haijun;

  • 作者单位

    Jiangxi Sci &

    Technol Normal Univ Sch Pharm Jiangxi Univ Fine Chem Ctr Engn Nanchang 330013 Jiangxi Peoples R China;

    Jiangxi Sci &

    Technol Normal Univ Sch Pharm Jiangxi Univ Fine Chem Ctr Engn Nanchang 330013 Jiangxi Peoples R China;

    Jiangxi Sci &

    Technol Normal Univ Sch Pharm Jiangxi Univ Fine Chem Ctr Engn Nanchang 330013 Jiangxi Peoples R China;

    Jiangxi Sci &

    Technol Normal Univ Sch Pharm Jiangxi Univ Fine Chem Ctr Engn Nanchang 330013 Jiangxi Peoples R China;

    Jiangxi Sci &

    Technol Normal Univ Sch Pharm Jiangxi Univ Fine Chem Ctr Engn Nanchang 330013 Jiangxi Peoples R China;

    Jiangxi Sci &

    Technol Normal Univ Sch Pharm Jiangxi Univ Fine Chem Ctr Engn Nanchang 330013 Jiangxi Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 无机化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号