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首页> 外文期刊>International endodontic journal >A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment
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A systematic review of methods used to sample and analyse periradicular tissue fluid during root canal treatment

机译：用于在根管治疗过程中对肝脏组织液进行采样和分析肝癌的系统综述

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Abstract Aim The primary aim was to identify techniques used to sample and analyse periradicular tissue fluid ( PTF ) in permanent teeth diagnosed with apical disease during root canal treatment. Secondly, to identify the types of inflammatory mediators studied using this approach. Methodology Data Sources: PubMed, EMBASE , Cochrane Library, Science Direct, Web of Science and OpenGrey. Eligibility Criteria : Clinical studies published until 1 June 2018 which utilized orthograde techniques to sample and analyse PTF were included. Cell culture, laboratory or animal studies and those concerned with investigating inflammatory mediator activity from within healthy or diseased pulp tissue, and not periradicular tissues, were excluded. Study appraisal and methods: In accordance with PRISMA guidelines, data were extracted on study characteristics, target mediators, sampling and assay techniques and the parameters associated with the PTF sampling and eluting protocol. A qualitative synthesis was conducted, and studies were critically appraised using a modified version of the Cochrane risk of bias tool. Results Study Characteristics: From 251 studies, 33 were eligible for inclusion. Sampling techniques included the use of paper points ( n? =?27), fine needle aspiration ( n? =?4) and filter strips ( n? =?2). Assay techniques included enzyme‐linked immunosorbent assay ( n? =?18), quantitative polymerase chain reaction ( n? =?9), radioimmunoassay ( n? =?4), colorimetric assay ( n? =?2), immunofluorometric assay ( n? =?1) and cytometric bead array ( n? =?1). Forty‐five different inflammatory mediators were targeted at the proteomic/metabolomic ( n? =?25) or transcriptomic level ( n? =?9). Limitations: Significant heterogeneity exists within the methodology, and only 5 studies disclosed unambiguous information about their PTF sampling and eluting protocols. Conclusions Paper points and proteomic/metabolomic analysis are currently the preferred methods for studying and analysing PTF during root canal treatment. The most studied analytes were IL ‐1β and TNF ‐α. Implications: Further research is required to develop an optimized PTF sampling and eluting protocol to overcome methodological heterogeneity, and future studies are advised to follow a standardized approach to reporting their methodology.
机译：摘要旨在识别用于在根管治疗期间诊断出诊断出具有顶端疾病的永久性牙齿的常见牙齿的技术和分析常见牙齿的技术。其次，为了鉴定使用这种方法研究的炎症介质的类型。方法数据来源：PubMed，Embase，Cochrane图书馆，科学直接，科学网站和OpenGrey。资格标准：临床研究发表于2018年6月1日，其中包括对外语进行样品和分析PTF的技术。排除了细胞培养，实验室或动物研究以及涉及从健康或患病的纸浆组织中调查炎症介质活性的人，而不是泊泊位组织。研究评估和方法：按照PRISMA指南，在研究特征，靶介质，采样和测定技术和与PTF采样和洗脱方案相关的参数上提取数据。进行了定性的合成，并且使用偏置工具的Cochrane风险的修改版本严重评估。结果研究特征：从251项研究，33名有资格包含。采样技术包括使用纸点（n？=Δ27），细针抽吸（n？=Δ4）和过滤条（n？=Δ2）。测定技术包括酶联免疫吸附测定（N？=β18），定量聚合酶链反应（n？=Δ9），放射免疫测定（n？=Δ4），比色测定（n？=Δ2），免疫荧光测定法（ n？=α1）和细胞计数珠阵列（n？=Δ1）。在蛋白质组学/代谢物（n？=Δ25）或转录组水平（n？= 9）中靶向四十五种不同的炎症介质。限制：方法中存在显着的异质性，并且只有5项研究披露了关于其PTF采样和洗脱协议的明确信息。结论纸张点和蛋白质组学/代谢物分析是目前在根管治疗过程中研究和分析PTF的首选方法。最多研究的分析物是IL-1β和TNF-α。含义：进一步的研究是开发优化的PTF采样和洗脱议定书，以克服方法学异质性，建议未来的研究遵循标准化的方法来报告其方法。

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