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首页> 外文期刊>International immunopharmacology >Protective role of liriodendrin in mice with dextran sulphate sodium-induced ulcerative colitis
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Protective role of liriodendrin in mice with dextran sulphate sodium-induced ulcerative colitis

机译：Liriodendrin在用葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠小鼠的保护作用

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摘要

Abstract Sargentodoxa cuneata , containing syringaresinol and its glycoside liriodendrin as the main bioactive compounds, is a well-known traditional Chinese medicine for treating intestinal inflammation. In our preliminary study, liriodendrin inhibited NF-kB activation in sepsis-induced acute lung injury. The present study was designed to investigate its effect on dextran sulfate sodium (DSS)-induced colitis in a mouse model and to explore the possible related mechanisms. Experimental colitis was established by giving mice drinking water containing 3% (w/v) DSS for 7days. The mice were pretreated with liriodendrin (100mg/kg/day, intragastrically) 3days before DSS treatment. We determined the effects of liriodendrin on disease activity index (DAI), colon length, histopathological examination, antioxidants, and anti-inflammatory activities. Our results showed that liriodendrin greatly decreased MPO and MDA activities and significantly increased SOD and GPx activities in the colon. Moreover, liriodendrin improved DAI, colon length and histological damage in colon and reduced the levels of pro-inflammatory cytokines, such as TNF-a, IL-1β and IL-6. Meanwhile, assessments by western blot revealed that liriodendrin significantly suppressed the activation of Akt and NF-κB pathways and up-regulated the expression of ERβ in the colon. In vitro, liriodendrin down-regulated production of pro-inflammatory cytokines and suppressed NF-κB signalling pathways in LPS-induced RAW 264.7 macrophages in a concentration-dependent manner. In addition, syringaresinol, the hydrolysate of liriodendrin, more potently down-regulated production of pro-inflammatory cytokines and suppressed NF-κB and Akt signalling pathways in LPS-induced RAW 264.7 macrophages,which were abolished by using a pure ER antagonist, ICI182, 780. Taken together, liriodendrin-mediated suppression of inflammatory damage in the colon may be attributable to the in vivo transformation to syringaresinol and liriodendrin may be a promising therapeutic approach preventive agent for colitis treatment. Highlights ? Liriodendrin was purified from a well-known traditional Chinese medicine for treating abdominal inflammation. ? The protective effect of liriodendrin on the ulcerative colitis was investigated for the first time. ? We revealed a novel mechanism underlying liriodendrin's anti-inflammatory and antioxidant effects.
机译：摘要Sargentodoxa Cuneata，含有含油索尼醇及其糖苷LioDendrin作为主要的生物活性化合物，是一种众所周知的中药，用于治疗肠炎症。在我们的初步研究中，LirioDendrin在败血症诱导的急性肺损伤中抑制了NF-KB活化。本研究旨在探讨其对小鼠模型中的硫酸硫酸葡聚糖钠（DSS）的影响，并探讨可能的相关机制。通过给予含有3％（w / v）DSS的小鼠饮用水来建立实验性结肠炎。在DSS治疗之前，用LirioDendrin（100mg / kg /天，胃地性）3天预处理小鼠。我们确定Liriodendrin对疾病活动指数（DAI），结肠长度，组织病理学检查，抗氧化剂和抗炎活动的影响。我们的研究结果表明，LirioDendrin大大降低了MPO和MDA活动，并在结肠中显着增加了SOD和GPX活动。此外，LirioDendrin改善了结肠癌，结肠长度和组织学损伤，并降低了促炎细胞因子的水平，例如TNF-A，IL-1β和IL-6。同时，Western印迹的评估显示，LiaDendrin显着抑制了AKT和NF-κB途径的激活，并上调了结肠中ERβ的表达。体外，LirioDendrin下调促炎细胞因子的产生，并抑制LPS诱导的原料264.7巨噬细胞的NF-κB信号传导途径以浓度依赖性方式。此外，Liodendrin的水解产物，LIS-诱导的原料264.7巨噬细胞的促炎细胞因子和抑制NF-κB和AKT信号通路的肝脏蛋白，更易于下调的NF-κB和AKT信号传导途径。 780.一起占用，LirioDendrin介导的结肠中炎性损伤的抑制可归因于对丁香酚醇的体内转化，并且LirioDendrin可能是结肠炎治疗的有望的治疗方法预防剂。强调 ？从众所周知的中药中纯化LirioDendrin，用于治疗腹部炎症。还LirioDendrin对溃疡性结肠炎对溃疡性结肠炎的保护作用。还我们揭示了LirioDendrin抗炎和抗氧化作用的新机制。

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  • 来源
    《International immunopharmacology》 |2017年第2017期|共8页
  • 作者

    Zhen Zhang; Lei Yang; Botao Wang; Lanqiu Zhang; Qi Zhang; Dihua Li; Shukun Zhang; Hongwei Gao; Ximo Wang;

  • 作者单位

    Graduate School of Tianjin Medical University;

    Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine;

    Graduate School of Tianjin Medical University;

    Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine;

    Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine;

    Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine;

    Tianjin Institute of Acute Abdominal Disease of Integrated Traditional Chinese and Western Medicine;

    Department of Anesthesiology Perioperative &

    Pain Medicine Brigham and Women's Hospital Harvard;

    Graduate School of Tianjin Medical University;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药理学;
  • 关键词

    Liriodendrin; Antioxidants; Anti-Inflammatory activities; Ulcerative colitis; ERβ;

    机译：LirioDendrin;抗氧化剂;抗炎活动;溃疡性结肠炎;ERβ;

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