• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Biotechnology and Biochemistry >Purification and Characterization of Xylanase from Aspergillus niger AK10 Cultivated on Rice Bran SolidState Medium
【24h】

Purification and Characterization of Xylanase from Aspergillus niger AK10 Cultivated on Rice Bran SolidState Medium

机译：米糠固体培养基中栽培的曲霉Ak10纯化与表征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Xylanase from Aspergillus niger AK10 was purified through two steps of column chromatography; namely, CM-Sephadex C50 ion-exchanger eluted with a gradient of 0-0. 5 M NaCl, and the other was Sephadex G-100 gel filtration. Its molecular weight as determined from SDS PAGE was 57. 4 kDa. The enzyme showed maximum activity at pH6 and at a temperature of 50°C. It could also maintain its activity at higher than 90% of the original level after 2-hr storage of the enzyme aliquot at either pH 5-6 or the temperatures of 50HC to 60°C. AK10 xylanase was sensitive at different levels to the inorganic ions tested, namely Mn2+, Zn2+, Cu2+, K+, Mg2; Co2+ and Fe2+. Crude enzyme extract from AK10 cultivated on rice bran solid medium containing 186. 5 U/ml of xylanase activity was used for preparing reducing sugar from leave litter. The hydrolyzate contained 1. 63 mg/ml of reducing sugar was found to be able to support growth of Escherichia coli. Bacillus subtilis and Pseudomonas aeruginosa in minimal medium. Thisrevealed the potential use of the enzyme in recycling agricultural wastes into fermentable sugar for production of microbial cells, metabolites and enzymes.
机译：通过柱色谱三个步骤纯化来自曲霉Ak10的木聚糖酶;即，CM-Sephadex C50离子交换器用0-0的梯度洗脱。 5M NaCl，另一个是Sephadex G-100凝胶过滤。从SDS页面测定的分子量为57. 4 KDA。酶在pH6和50℃的温度下显示出最大活性。在pH 5-6的酶等分试样或50HC至60℃的温度下，它还可以在高于原始水平的高于原始水平的90％的活性。 Ak10木聚糖酶对测试的无机离子的不同水平敏感，即Mn 2 +，Zn2 +，Cu 2 +，K +，Mg2; CO2 +和FE2 +。从含有1866的米糠固体培养基上培养的Ak10粗酶提取物。5 U / ml木聚糖酶活性用于制备废弃物的还原糖。含有1.63mg / ml的还原糖的水解产物被发现能够支持大肠杆菌的生长。枯草芽孢杆菌和铜绿假单胞菌在最小培养基中。这术反应了酶在回收农业废物中的潜在用途，以用于生产微生物细胞，代谢物和酶的发酵糖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号