Investigation of the potential active site of a cyanide dihydratase using site-directed mutagenesis

Atsushi Watanabe; Kazuyoshi Yano; Kazunori Ikebukuro; Isao Karube

首页> 外文期刊>Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology >Investigation of the potential active site of a cyanide dihydratase using site-directed mutagenesis

【24h】

Investigation of the potential active site of a cyanide dihydratase using site-directed mutagenesis

机译：使用定点诱变研究氰化物二水合酶的潜在活性位点

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Cyanide dihydratase has conserved residues in the amino acid sequence with nitrilase, and cyanide hydratase. The conserved amino acid residues in the cyanide dihydratase from Pseudomonas stutzeri AK61 were altered by site-directed mutagenesis. The enzyme completely lost its activity of the cyanide hydrolysis by the replacement of cysteine-163 to serine. The replacement of tyrosine-53 to phenylalanine caused an increase of the K_m value of the enzyme for cyanide. Substitution of nine other residues seemed to affect the structure of the enzyme.

机译：氰化物二水合酶与腈水解酶和氰化物水合酶在氨基酸序列中具有保守的残基。定点诱变改变了斯氏假单胞菌AK61氰化物二水合酶中的保守氨基酸残基。通过将半胱氨酸163替换为丝氨酸，该酶完全丧失了氰化物水解的活性。将酪氨酸53替换为苯丙氨酸导致氰化物酶的K_m值增加。其他9个残基的取代似乎会影响酶的结构。

著录项

来源
《Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology》 |1998年第0期|共4页
作者
Atsushi Watanabe; Kazuyoshi Yano; Kazunori Ikebukuro; Isao Karube;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物化学;分子生物学;
关键词
cyanide dihydratase; site-directed mutagenesis; active site; enzyme activity; nitrilase;

机译：氰化物二水合酶;定点诱变;活性位;酶活性;硝化酶;

相似文献

外文文献
中文文献
专利

1. Investigation of the potential active site of a cyanide dihydratase using site-directed mutagenesis. [J] . Watanabe A, Yano K, Ikebukuro K, Biochimica et biophysica acta: international journal of biochemistry and biophysics . 1998,第1期

机译：使用定点诱变研究氰化物二水合酶的潜在活性位点。
2. Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme [J] . Wilderman P. Ross, Gay Sean C., Jang Hyun-Hee, The FEBS journal . 2012,第9期

机译：通过定点诱变研究基于配体结合酶的晶体结构的细胞色素P450 2B4非活性位点残基在蛋白质-配体相互作用中的作用
3. Site-directed mutagenesis of the active site glutamate in human matrilysin: investigation of its role in catalysis [J] . Cha J, Auld DS Biochemistry . 1997,第50期

机译：活性位点谷氨酸在人类基质溶素中的定点诱变：在催化作用中的作用研究
4. β-D-Xylosidase from Selenomonas ruminantium: Role of Glutamate 186 in Catalysis Revealed by Site-Directed Mutagenesis, Alternate Substrates, and Active-Site Inhibitor [C] . Douglas Brian Jordan, Jay D. Braker Symposium on biotechnology for fuels and chemicals . 2010

机译：来自Selenomonas强溶酪蛋白的β-D-木糖苷酶：谷氨酸186在催化中的作用，通过定点诱变，交替底物和活性位点抑制剂揭示
5. Aristolochene synthase: Probing the role of active site amino acids by site-directed mutagenesis. [D] . Felicetti, Brunella. 2004

机译：马兜铃合酶：通过定点诱变探索活性位氨基酸的作用。
6. Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme [O] . P. Ross Wilderman, Sean C. Gay, Hyun-Hee Jang, -1

机译：基于配体酶晶体结构的蛋白质 - 配体相互作用的细胞色素P450 2B4非活性位点残基的作用研究
7. Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis [O] . Emine Mattern-Dogru, Xueyan Ma, Joachim Hartmann, 2002

机译：潜在的活性位点残留在多氨氨酸醛酯酶中，吲哚生物合成的中央酶，通过建模和定点诱变

Investigation of the potential active site of a cyanide dihydratase using site-directed mutagenesis

摘要

著录项

相似文献

相关主题

期刊订阅