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首页> 外文期刊>International journal of ecohydrology and hydrobiology >The occurrence of antibiotic-resistance genes in the Pilica River, Poland
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The occurrence of antibiotic-resistance genes in the Pilica River, Poland

机译:Pland河中抗生素抗生素基因的发生

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The aim of study was to analyze the occurrence of antibiotic-resistance genes (ARGs) in the Pilica River. Seasonal variations in the occurrence of ARGs in river water were also determined. Water samples were collected in 7 sites situated along a 186-km section of the Pilica River from Przedborz to estuary. River water was sampled four times between November 2017 and July 2018. Environmental DNA was isolated from water samples, and selected genes encoding resistance to tetracyclines (tet(A), tet(B), tet(C), tet(D), tet(E), beta-lactams (bla(OXA), bla(SHV), bla(TEM), blu(CTX-M)), macrolide, lincosamide and streptogramin (MLS) antibiotics (ermF and linA), sulfonamides (sull), fluoroquinolones (aac(6')-Ib-a) as well as the integrase 1 (intil) gene and the 16S rRNA gene were amplified by standard PCR. ARGs were most prevalent and diverse in winter samples which harbored the intl1 gene and genes encoding resistance to beta-lactams, MLS antibiotics, sulfonamides and tetracyclines. The bla(OXA) and bla(CTX-M) genes were detected only in two autumn samples. The bla(TEM) gene was prevalent in all samples regardless of the time and place of sampling. Variations in the prevalence of ARGs were not observed along the analyzed section of the Pilica River, which indicates that pollutant loads did not increase along the river continuum. It should be noted that selected genes (bla(TEM), tet(A), tet(E), ermF, linA) were present in all or nearly all water samples regardless of sampling date or sampling site, which indicates that these ARGs are ubiquitous in the environment. (C) 2019 European Regional Centre for Ecohydrology of the Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.
机译:研究的目的是分析Pilica河流中抗生素抗性基因(Args)的发生。还确定了河水中args发生的季节性变化。在沿着Przedborz到河口的Pilica河的186公里段,在7位点收集水样。 2017年11月和2018年7月在2018年7月期间采样了四次。从水样中分离出环境DNA,以及编码对四环素的抗性的选定基因(TET(A),TET(B),TET(C),TET(D),TET (e),β-内酰胺(BLA(OXA),BLA(SHV),BLA(TEM),BLU(CTX-M)),大环内酯,林磷酰胺和链图(MLS)抗生素(ERMF和LINA),磺胺酰胺(SULL) ,通过标准PCR扩增氟代喹啉(AAC(6') - IB-A)以及整合酶1(INTLA)基因和16S rRNA基因。args在冬季样本中最普遍,多样化,患有INTL1基因和基因编码对β-内酰胺,MLS抗生素,磺酰胺和四环素的抗性。仅在两个秋季样品中检测到BLA(OXA)和BLA(CTX-M)基因。无论时间内,BLA(TEM)基因都在所有样品中普遍存在和抽样的地方。沿着Pilica河的分析部分观察到args患病率的变化,这表明污染物负荷没有进入沿着河沿着河连连线折痕。应该注意的是,无论采样日期还是抽样站点,所有或几乎所有的水样都存在所选基因(BLA(TEM),TET(A),TET(E),TET(E),ERMF,LINA),这表明这些args是普遍存在的环境中。 (c)2019年欧洲区域地区生态学研究中心的波兰科学院。 elsevier b.v出版。保留所有权利。

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