...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Genomics >Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers
【24h】

Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers

机译：乌克希尔菌的差异微小罗纳分析Pseudomallei-和Francisella Tullensis暴露的HPBMCs揭示了潜在的生物标志物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Increasing evidence that microRNAs (miRNAs) play important roles in the immune response against infectious agents suggeststhat miRNA might be exploitable as signatures of exposure to specific infectious agents. In order to identify potential earlymiRNA biomarkers of bacterial infections, human peripheral blood mononuclear cells (hPBMCs) were exposed to two selectagents, Burkholderia pseudomallei K96243 and Francisella tularensis SHU S4, as well as to the nonpathogenic controlEscherichia coli DH5α. RNA samples were harvested at three early time points, 30, 60, and 120 minutes postexposure, thensequenced. RNAseq analyses identified 87 miRNAs to be differentially expressed (DE) in a linear fashion. Of these, 31 miRNAswere tested using the miScript miRNA qPCR assay. Through RNAseq identification and qPCR validation, we identifieddifferentially expressed miRNA species that may be involved in the early response to bacterial infections. Based upon itsupregulation at early time points postexposure in two different individuals, hsa-mir-30c-5p is a miRNA species that could bestudied further as a potential biomarker for exposure to these gram-negative intracellular pathogens. Gene ontology functionalanalyses demonstrated that programmed cell death is the first ranking biological process associated with miRNAs that areupregulated in F. tularensis-exposed hPBMCs.
机译：越来越多的证据表明微小RNA（miRNA）在免疫反应中发挥重要作用，免疫反应促使MIRNA可能被解释为暴露于特异性传染病的签名。为了鉴定细菌感染的潜在早期生物标志物，将人周围血液单核细胞（HPBMC）暴露于两种选择，Burkhowderia Pseudomallei K966243和Francisella Tularensis Shu S4以及非对照组ColiDH5α。然后在曝光后的三个早期时间点，30,60和120分钟收获RNA样品，然后连续。 RNASEQ分析确定了87个miRNA以线性方式差异表达（de）。其中，使用MIScript miRNA QPCR测定测试了31 mirnaswere。通过RNASEQ鉴定和QPCR验证，我们确定了可以参与细菌感染的早期反应的细节表达的miRNA物种。基于在两种不同个体的早期时间点的初始点的基础上，HSA-miR-30c-5p是一种miRNA物种，其可以进一步如潜在的生物标志物，以暴露于这些革兰氏阴性细胞内病原体。基因本体官能团证明，编程的细胞死亡是与MIRNA相关的第一排名生物过程，其在F.Tularensis暴露的HPBMC中令人生畏。

著录项

  • 来源
    《International Journal of Genomics》 |2017年第2期|共13页
  • 作者

    Regina Z. Cer; J. Enrique Herrera-Galeano; Kenneth G. Frey;

  • 作者单位

    Genomics and Bioinformatics Department Biological Defense Research Directorate Naval Medical Research Center Frederick MD USA;

    Genomics and Bioinformatics Department Biological Defense Research Directorate Naval Medical Research Center Frederick MD USA;

    Genomics and Bioinformatics Department Biological Defense Research Directorate Naval Medical Research Center Frederick MD USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
  • 关键词

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号