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首页> 外文期刊>International journal of developmental neuroscience: the official journal of the International Society for Developmental Neuroscience >Ontogeny of white matter, toll-like receptor expression, and motor skills in the neonatal ferret
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Ontogeny of white matter, toll-like receptor expression, and motor skills in the neonatal ferret

机译：白种的组织发生，偶然的受体表达，以及新生儿雪貂中的运动技能

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Inflammation caused by perinatal infection, superimposed with hypoxia and/or hyperoxia, appears to be important in the pathogenesis of preterm neonatal encephalopathy, with white matter particularly vulnerable during the third trimester. The associated inflammatory response is at least partly mediated through Toll-like receptor (TLR)-dependent mechanisms. Immunohistochemistry, gene expression, and behavioral studies were used to characterize white matter development and determine TLR3 and TLR4 expression and accumulation in the neonatal ferret brain. Expression of markers of white matter development increased significantly between postnatal day (P)1 and P10 (NG2, PDGFRα) or P15 (Olig2), and either remained elevated (NG2), or decreased again at P40 (PDGFRα, Olig2). Olig2 immunostaining within the internal capsule was also greatest at P15. Myelin basic protein (MBP) immunostaining and mRNA expression increased markedly from P15 to P40 and into adulthood, which correlated with increasing performance on behavioral tests (negative geotaxis, cliff aversion, righting reflex, and catwalk gait analysis). TLR4 and TLR3 positive staining was low at all ages, but TLR3 and TLR4 mRNA expression both increased significantly from P1 to P40. Following lipopolysaccharide (LPS) and hypoxia/hyperoxia exposure at P10, meningeal and parenchymal inflammation was seen, including an increase in TLR4 positive cells. These data suggest that the neuroinflammation associated with prematurity could be modeled in the newborn ferret.
机译：围产期感染引起的炎症呈缺氧和/或高氧因叠加，似乎在早产新生儿脑病的发病机制中都很重要，白质特别易受在三个三个月的伤害。相关的炎症反应至少部分地通过Toll样受体（TLR）依赖性机制介导。使用免疫组织化学，基因表达和行为研究来表征白土发育并确定新生儿雪貂脑中的TLR3和TLR4表达和积累。白质发育的表达在产后产后（P）1和P10（NG2，PDGFRα）或P15（OLIG2）之间显着增加，并且仍然升高（NG2），或在P40（PDGFRα，olig2）再次下降。内囊内的OLIG2免疫染色在P15也最大。髓鞘碱性蛋白质（MBP）免疫染色和mRNA表达显着从P15到P40和成年期增加，与增加的行为试验的性能相关（负土工睫毛，悬崖厌厌，抗反射和潮汐步态分析）相关。 TLR4和TLR3在所有年龄段的阳性染色低，但TLR3和TLR4 mRNA表达两者都从P1到P40显着增加。在P10，脑膜炎和实质炎症下进行脂多糖（LPS）和缺氧/高氧暴露，包括TLR4阳性细胞的增加。这些数据表明，与早产相关的神经炎症可以在新生雪貂中进行建模。

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