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首页> 外文期刊>International Journal of Horticulture & Crop Science Research >Effect of Light Quality on Chlorophyll Accumulation and Protein Expression in Wheat (Triticum aestivum L.) Seedlings
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Effect of Light Quality on Chlorophyll Accumulation and Protein Expression in Wheat (Triticum aestivum L.) Seedlings

机译：光质对小麦叶绿素积累和蛋白质表达的影响（Triticum Aestivum L.）幼苗

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摘要

Light is a very important environmental factor which affects plant growth and development in various important ways. Not only quality of light but quantity of light is also very important in determining the growth of plants therefore the present studywas aimed to analyze the growth of wheat seedlings in different light qualities. Wheat seedlings grown on germination paper under high intensity red light (400 umoles m"2 s"1) were yellowish-white in color and accumulated minimum amounts of chlorophylland carotenoid. However wheat seedlings when grown in dark were etiolated and when grown under different light conditions, as high intensity red light in vermiculite, red +blue, far-red, red + far-red, and white light were green in color and accumulateddifferent amounts of chlorophyll and carotenoids. 2D-PAGE of total protein isolated from plants grown under different light regimes showed absence of many proteins in red light grown non-green seedlings and etiolated seedlings as compared to green seedlings. All the green plants grown under different light conditions showed steady state expression of many proteins. These seedlings also accumulated many light quality specific proteins, i.e., those proteins were only present in a particular light environment and absent in the other. Western blot analysis of Rubisco LSU, OEC33, and Cyt-f showed reduced abundance in red light grown non-green seedlings but were abundantly present in green seedlings. POR was accumulated in etiolated seedlings and reduced inother light grown seedlings. The results show that light quality is a very important environmental factor in influencingchlorophyll content as well as expression of other protein and is an important factor affecting plants growth under different areas receiving different qualities of the light.
机译：光线是一种非常重要的环境因素，以各种重要的方式影响植物生长和发展。不仅光的质量，而且光量在确定植物的生长方面也非常重要，因此目前的研究旨在分析不同光质品质的小麦幼苗的生长。在高强度红光下萌发纸上种植的小麦幼苗（400 umoles m“2 s”1）是黄白色的呈黄白色，累积最低量的叶绿素类胡萝卜素。然而，当在黑暗中生长时，小麦幼苗在不同的光线条件下生长时，在蛭石中的高强度红光，红色+蓝色，远红色，红色+远红色，白光是绿色的，呈绿色和累积量叶绿素和类胡萝卜素。与不同光长的植物中分离的植物中分离的总蛋白质的2D-Page显示出红光生长非绿色幼苗和青光幼苗的许多蛋白质的缺失，与绿色幼苗相比。在不同光线条件下种植的所有绿色植物都显示出许多蛋白质的稳态表达。这些幼苗还积累了许多轻质质量的特异性蛋白质，即，这些蛋白质仅存在于特定光环境中并且在另一个蛋白质中缺席。 Rubisco LSU，OEC33和CYT-F的Western印迹分析显示，红光生长非绿色幼苗的丰度降低，但在绿色幼苗中大量存在。 POR累积在硫酸化幼苗中，并减少了来自其他光生长的幼苗。结果表明，光质质量是影响氯苯含量的非常重要的环境因素以及其他蛋白质的表达，是影响植物生长在接受不同品质的不同区域下的植物生长的重要因素。

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