...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of medical microbiology: IJMM >Virulence-related genes are associated with clinical and nutritional outcomes of Shigella/Enteroinvasive Escherichia coli pathotype infection in children from Brazilian semiarid region: A community case-control study
【24h】

Virulence-related genes are associated with clinical and nutritional outcomes of Shigella/Enteroinvasive Escherichia coli pathotype infection in children from Brazilian semiarid region: A community case-control study

机译：毒力相关基因与巴西半干旱地区儿童的志贺氏菌/肠内大肠杆菌病理型感染的临床和营养结果有关：社区案例控制研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Shigella/Enteroinvasive Escherichia coli (EIEC) pathotype is a major enteropathogen associated with diarrhea and malnutrition in children from developing countries. This study aimed to correlate Shigella/EIEC virulence-related genes (VRGs) with clinical symptoms, nutritional status and coenteropathogens in children from the Brazilian semiarid region. We designed a case-control study of community diarrhea in six cities of the Brazil semiarid region with 1200 children aging 2-36 months. Standardized questionnaire was applied for collecting sociodemographic, nutritional status and clinical information of the children. DNA samples were extracted from stools and diagnosed for Shigella/EIEC using PCR-based approaches. Positive samples were tested for 28 VRGs using four multiplex PCRs. Intestinal inflammation was determined by measuring fecal myeloperoxidase (MPO). Shigella/EIEC pathotype was detected in 5% of the children and was significantly associated with diarrhea. The genes sen (encoding Shigella enterotoxin 2), ipgB2, ipgB1 (both encoding type 3 secretion system-T3SS effectors that modulate actin filament), and ospF (encoding a T3SS effector involved in suppression of host responses) were further associated with diarrhea in Shigella/EIEC positive children. Among children presenting diarrhea, virA gene (encoding a T3SS effector that promotes microtubule destabilization) was associated with fever, while virB (encoding a major transcriptional activator) was associated with low height-for-age z-score. In addition, these VRGs were associated with increased fecal MPO, and coinfection with Salmonella spp. was associated with increased abdominal pain. These data reinforce the impact of Shigella/EIEC on diarrhea in children from Brazilian semiarid region and highlighted the contributions of specific virulence genes for its pathobiology.
机译：Shigella /肠内侵入性大肠杆菌（EIEC）致病型是与发展中国家的儿童腹泻和营养不良有关的主要肠球病。本研究旨在将志贺氏菌/ eIEC毒力相关基因（VRG）与巴西半干旱地区的儿童中的临床症状，营养状况和结合剂相关联。我们设计了对巴西半干旱地区六个城市的社区腹泻的病例对照研究，1200名儿童2-36个月。标准化的问卷适用于收集儿童的社会造影，营养状况和临床信息。用基于PCR的方法从粪便中萃取DNA样品并诊断为Shigella / EIEC。使用四种多重PCRS测试28 VRG的阳性样品。通过测量粪便髓过氧化物酶（MPO）测定肠炎症。在5％的儿童中检测到Shigella / EiC型病理型，与腹泻显着相关。该基因SEN（编码志氏肠毒素2），IPGB2，IPGB1（编码3型分泌系统-T3SS效应器调节肌动蛋白长丝的效应器）和OSPF（编码抑制宿主反应的T3SS效应）进一步与令人遗叫的腹泻有关/ eiec阳性儿童。在患有腹泻的儿童中，Vira基因（编码促进微管稳定化的T3SS效应）与发热有关，而病毒（编码主要转录激活剂）与低高度Z分数相关。此外，这些VRG与粪便增加有关，与沙门氏菌SPP的繁殖。与增加的腹痛有关。这些数据强化了志贺氏菌/ eIEC在巴西半干旱地区儿童腹泻的影响，并突出了特定毒力基因对其病理学的贡献。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号