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首页> 外文期刊>Israel Journal of Plant Sciences >Justicia beddomei, a source of comprehensive vasicinone production
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Justicia beddomei, a source of comprehensive vasicinone production

机译：Justicia Beddomei，综合旋转腺松生产的来源

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摘要

The present study formulates a method for comprehensive production of vasicinone, a quinazoline alkaloid, from multiple plant parts of in vitro and in-field-grown Justicia beddomei. HPTLC analysis of plant parts was executed with methanolic extract using toluene: butanol: butyl acetate (9:0.5:0.5; v/v/v) as the solvent system. Validation of methodology was accomplished using TLC plates (silica gel 60 F254-pre-coated aluminium sheet) following the ICH manual to maintain accuracy, precision and repeatability with a linearity ranging 2-6 mu g/spot. Validation data offers precision to the methodology adapted in the present study (LOD 1 mu g/spot and LOQ 3 mu g/spot). It was evident that in vitro samples produced relatively higher levels of vasicinone than that of their in-field counterparts. The highest vasicinone (2.07 +/- 0.025% of dry weight) production was quantified from in vitro stem, signifying a new resource for the production of vasicinone from identified parts of in vitro and in-field propagated J. beddomei plants.
机译：本研究制定了一种用于综合喹啉酮，喹唑啉生物碱的综合生产的方法，来自多种植物部分的体外和现场生长的真生床单。使用甲苯的甲烷提取物进行植物零件的HPTLC分析：丁醇：乙酸丁酯（9：0.5：0.5; v / v / v）作为溶剂体系。使用TLC板（硅胶60F254-预涂覆的铝板）完成了方法的验证，以保持精度，精度和可重复性，其线性测量为2-6μg/斑。验证数据为适用于本研究的方法（LOD 1 MU G / SPOT和LOQ3μg/斑点）提供精确度。显然，在体外样品产生比其现场对应物的含量相对较高。从体外茎量化了最高的血压蛋白（2.07 +/- 0.025％的干重）产生，从体外和现场繁殖的J.Demdomei植物中鉴定了从鉴定的群体生产的唾液腺生产的新资源。

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