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首页> 外文期刊>Insect Biochemistry and Molecular Biology >Genome-wide annotation and comparative analysis of cuticular protein genes in the noctuid pest Spodoptera litura
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Genome-wide annotation and comparative analysis of cuticular protein genes in the noctuid pest Spodoptera litura

机译：Noctuid Pest Spodoptera Litura基因组宽注释和细胞蛋白基因的比较分析

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摘要

Insect cuticle is considered an adaptable and versatile building material with roles in the construction and function of exoskeleton. Its physical properties are varied, as the biological requirements differ among diverse structures and change during the life cycle of the insect. Although the bulk of cuticle consists basically of cuticular proteins (CPs) associated with chitin, the degree of cuticular sclerotization is an important factor in determining its physical properties. Spodoptera litura, the tobacco cutworm, is an important agricultural pest in Asia. Compared to the domestic silkworm, Bombyx mori, another lepidopteran whose CP genes have been well annotated, S. litura has a shorter life cycle, hides in soil during daytime beginning in the 5th instar and is exposed to soil in the pupal stage without the protection of a cocoon. In order to understand how the CP genes may have been adapted to support the characteristic life style of S. litura, we searched its genome and found 287 putative cuticular proteins that can be classified into 9 CP families (CPR with three groups (RR-1, RR-2, RR-3), CPAP1, CPAP3, CPF, CPFL, CPT, CPG, CPCFC and CPLCA), and a collection of unclassified CPs named CPH. There were also 112 cuticular proteins enriched in Histidine residues with content varying from 6% to 30%, comprising many more His-rich cuticular proteins than B. mori. A phylogenetic analysis between S. litura, M. sexta and B. mori uncovered large expansions of RR-1 and RR-2 CPs, forming large gene clusters in different regions of S. litura chromosome 9. We used RNA-seq analysis to document the expression profiles of CPs in different developmental stages and tissues of S. litura. The comparative genomic analysis of CPs between S. litura and B. mori integrated with the unique behavior and life cycle of the two species offers new insights into their contrasting ecological adaptations.
机译：昆虫角质层被认为是一种适应性和多功能的建筑材料，具有施工和功能的作用。它的物理性质是多样的，因为生物要求在各种结构中不同，并且在昆虫的生命周期期间变化。尽管面包杆菌基本上由与甲壳素相关的切割蛋白质（CPS）组成，但有切割的硬化程度是确定其物理性质的重要因素。 Spodoptera Litura，烟草植物，是亚洲重要的农业害虫。与家蚕相比，Bombyx Mori，另一种鳞翅目，其另一种鳞翅目，其CP基因已充分注释，S. Litura具有较短的生命周期，在第5次开始的白天在白天覆盖土壤，并在没有保护的情况下暴露于蛹阶层的土壤。没有保护一个茧。为了了解CP基因如何适应S. Litura的特征生活方式，我们搜查了其基因组，发现了287个推定的蛋白质，可分为9个CP家族（CPR三组（RR-1 ，RR-2，RR-3），CPAP1，CPAP3，CPF，CPFL，CPT，CPG，CPCFC和CPLCA）以及名为CPH的未分类CP的集合。在组氨酸残基中还有112种蛋白质富含含量的蛋白质，其含量不同于6％至30％，包含许多富含他丰富的蛋白质蛋白质而不是B. Mori。 S. Litura，M.Sexta和B. Mori之间的系统发育分析揭示了RR-1和RR-2 CP的大扩展，在S. Litura染色体的不同地区形成了大型基因簇。我们使用RNA-SEQ分析文件CPS在S. Litura不同发育阶段和组织中CPS的表达谱。与两种物种独特行为和生命周期相结合的S. Litura和B. Mori之间的CPS的比较基因组分析提供了对对比生态适应的新见解。

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    Liu Jianqiu; Li Shenglong; Li Wanshun; Peng Li; Chen Zhiwei; Xiao Yingdan; Gu Huizhen; Zhang Jiwei; Cheng Tingcai; Goldsmith Marian R.; Arunkumar Kallare P.; Xia Qingyou; Mita Kazuei;

  • 作者单位

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

    Southwest Univ Biol Sci Res Ctr State Key Lab Silkworm Genome Biol Chongqing 400716 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    Cuticular protein; Comparative analysis; Histidine-rich; RNA-Seq; Spodoptera litura;

    机译：切割蛋白;对比分析;富含组氨酸;RNA-SEQ;Spodoptera Litura;

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