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首页> 外文期刊>BJU international >Influence of blood sampling on protein profiling and pattern analysis using matrix-assisted laser desorption/ionisation mass spectrometry.
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Influence of blood sampling on protein profiling and pattern analysis using matrix-assisted laser desorption/ionisation mass spectrometry.

机译：血液采样对使用基质辅助激光解吸/电离质谱的蛋白质谱分析和模式分析的影响。

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OBJECTIVE: To describe the influence of blood sampling/sampling tubes on mass spectrometric and clustering results, and on clinical blood variables, in blood samples collected from healthy volunteers and patients with prostate cancer. PATIENTS, SUBJECTS AND METHODS: Two venous blood samples were taken from 12 healthy volunteers and 12 patients with localized prostate cancer. Two blood samples were taken from each participant using two different venepuncture systems (group A and group B). The Kolmogorov-Smirnov test was used to identify the peaks distinguishing the different groups. In a 10-fold cross-validation study, decision trees for identifying discriminatory peaks that separate the benign from the malignant were constructed. RESULTS: The decision tree separated samples measured by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) from healthy volunteers from those of patients with prostate cancer, with a sensitivity of 93.6% and a specificity of 91.6%. Of special interest isthat one peak at 6941 m/z was produced during blood sample preparation and had a very powerful influence on the results of the classification. CONCLUSION: The results clearly showed that blood-sampling systems have a great influence on the recorded MALDI MS traces, and thus can markedly influence and confound the results of the MS analysis, whereas clinical variables might remain unchanged. MS profiling is a promising method of marker discovery, but as it could be shown well-designed studies are critical to allow proper interpretation for the identification of key variables as well as for the clinical use.
机译：目的：描述从健康志愿者和前列腺癌患者采集的血液样本中，采样/采样管对质谱和聚类结果以及临床血液变量的影响。患者，受试者和方法：从12位健康志愿者和12位局限性前列腺癌患者中抽取两个静脉血样本。使用两个不同的静脉穿刺系统（A组和B组）从每个参与者中采集两个血液样本。 Kolmogorov-Smirnov试验用于鉴定区分不同组的峰。在一项10倍交叉验证研究中，构建了用于识别将良性与恶性区分开的区分性峰的决策树。结果：决策树从前列腺癌患者的健康志愿者中分离出了通过基质辅助激光解吸/电离质谱法（MALDI MS）测量的样品，敏感性为93.6％，特异性为91.6％。特别令人感兴趣的是，在血样制备过程中产生了一个6941 m / z的峰，对分类结果有非常大的影响。结论：结果清楚地表明，血液采样系统对记录的MALDI MS痕迹有很大的影响，因此可以显着影响和混淆MS分析的结果，而临床变量可能保持不变。 MS谱图分析是一种很有前途的标记物发现方法，但可以证明，精心设计的研究对于正确解释关键变量以及临床应用至关重要。

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