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首页> 外文期刊>EMBO reports >PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development
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PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development

机译：PNLDC1，小鼠预分子修剪器，是减数分裂和后期后男性生殖细胞发展所必需的

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摘要

PIWI-interacting RNAs (piRNAs) are germ cell-specific small RNAs essential for retrotransposon gene silencing and male germ cell development. In piRNA biogenesis, the endonuclease MitoPLD/Zucchini cleaves long, single-stranded RNAs to generate 5 termini of precursor piRNAs (pre-piRNAs) that are consecutively loaded into PIWI-family proteins. Subsequently, these pre-piRNAs are trimmed at their 3'-end by an exonuclease called Trimmer. Recently, poly(A)-specific ribonuclease-like domain-containing 1 (PNLDC1) was identified as the pre-piRNA Trimmer in silkworms. However, the function of PNLDC1 in other species remains unknown. Here, we generate Pnldc1 mutant mice and analyze small RNAs in their testes. Our results demonstrate that mouse PNLDC1 functions in the trimming of both embryonic and post-natal pre-piRNAs. In addition, piRNA trimming defects in embryonic and post-natal testes cause impaired DNA methylation and reduced MIWI expression, respectively. Phenotypically, both meiotic and post-meiotic arrests are evident in the same individual Pnldc1 mutant mouse. The former and latter phenotypes are similar to those of MILI and MIWI mutant mice, respectively. Thus, PNLDC1-mediated piRNA trimming is indispensable for the function of piRNAs throughout mouse spermatogenesis.
机译：PIWI - 相互作用的RNA（PIRNA）是细胞特异性小RNA，其对转回转瘤基因沉默和男性生殖细胞发育。在PiRNA生物生成中，内切核酸酶Mitopld / Succhini切割长，单链RNA，以产生5个末端的前体PiRNA（预吡喃），其连续地装载到PiWi-Family蛋白中。随后，通过称为微调器的外切核酸酶在它们的3'-末端修剪这些预先染色的。最近，鉴定含有含有畴样域的1（PNLDC1）作为蚕食前的PiRNA修剪器。然而，其他物种中PNLDC1的功能仍然未知。在这里，我们生成PNLDC1突变小鼠并在其睾丸中分析小RNA。我们的结果表明，小鼠PNLDC1在胚胎和产后前PiRNA的修剪中起作用。此外，胚胎和产后睾丸中的PiRNA修剪缺陷分别导致DNA甲基化和细胞表达减少。表型，减少人和后后病人在同一个体PNLDC1突变小鼠中是明显的。前者和后一种表型分别与MILI和MIWI突变小鼠类似。因此，PNLDC1介导的PiRNA修剪对于PiRNA在小鼠精子发生过程中是必不可少的。

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