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Urea Biosensor Based on Electrochromic Properties of Prussian Blue

机译：基于普鲁士蓝电致变色特性的尿素生物传感器

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摘要

Prussian blue (PB) is an electrochromic material, which can be used as a signal transducer in the formation of optical urea biosensors. The previous researches in electrochromic properties of PB demonstrated the optical PB response to ammonium ions, which occurs when ammonium ions are interacting with PB layer at a constant 0.2 V vs Ag|AgCl|KClsat potential. In this work PB optical dependence on ammonium ions concentration was applied in the formation of electrochromic urea biosensor. Biosensor was formed by modifying the optically transparent indium tin oxide (ITO) coated glass electrode (glass/ITO) with Prussian blue layer and immobilizing urease (glass/ITO/PB-urease). Calibration curve showed the linear dependency (R-2=0.995) between the change of maximal absorbance (Delta A) and urea concentration in concentration range varying from 3 mM to 30 mM. The highest sensitivity (4 Delta A M-1) of glass/ITO/PB-urease biosensor is in the concentration range from 7 mM to 30 mM. It was determined that working principle of the glass/ITO/PB-urease biosensor is not related to pH changes occurring during enzymatic hydrolysis of urea.
机译：普鲁士蓝（PB）是一种电致变色材料，可用作光学尿素生物传感器的形成中的信号换能器。 PB电致变色性质的先前研究证明了对铵离子的光学PB反应，当铵离子以恒定0.2VVS Ag | KCLSAT电位与Pb层相互作用时发生。在这项工作中，在电致变色尿素生物传感器的形成中施加对铵离子浓度的光学依赖性。通过用普鲁士蓝层和固定释放脲酶（玻璃/ ITO / PB-脲酶）来改变光学透明铟锡（ITO）涂覆的玻璃电极（玻璃/ ITO）来形成生物传感器。校准曲线在最大吸光度（Delta A）的变化和浓度范围内不同3mm至30mm之间的尿素浓度之间显示线性依赖性（R-2 = 0.995）。玻璃/ ITO / PB-脲酶生物传感器的最高敏感性（4ΔM-1）的浓度范围为7mm至30mm。确定玻璃/ ITO / Pb-脲酶生物传感器的工作原理与尿素酶水解期间发生的pH发生的pH值不相关。

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