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首页> 外文期刊>Electroanalysis >Highly Sensitive in vitro Biosensor for Enterotoxigenic Escherichia coli Detection Based on ssDNA Anchored on PtNPs-Chitosan Nanocomposite
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Highly Sensitive in vitro Biosensor for Enterotoxigenic Escherichia coli Detection Based on ssDNA Anchored on PtNPs-Chitosan Nanocomposite

机译：基于SSDNA锚定对PTNPS-Chotosan纳米复合材料的SSDNA高度敏感的体外生物传感器

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Detection of Enterotoxigenic Escherichia coli in various biological samples has tremendous importance in human health. In this direction, we have designed a label free electrochemical biosensor for highly selective detection of Escherichia coli through detecting ST gene. The ability of sensor probe to detect STG was confirmed using polymerase chain reaction. The biosensor was fabricated based on STG specific probes immobilized on platinum nanoparticles chitosan nanocomposite on screen printed carbon electrode, which was characterized by cyclic voltammetry, transmission electron microscopy, and fourier transform infrared spectroscopy. A highly sensitive label free sensing was achieved by analyzing STG hybridization using electrochemical impedance spectroscopy (EIS) technique. The EIS analysis showed a significant increase in charge transfer resistance after STG interaction with the highly selective ssDNA probe immobilized on the nanocomposite film. The increase in charge transfer resistance was evaluated for varying concentrations of STG, which shows a dynamic range between 1.0x10(-12) and 1.0x10(-4) with the detection limit of 3.6x10(-14) M (RSD<4.5%). The regeneration of sensor probe was also studied and interference due to non-target sequences was evaluated to ensure the selectivity of the designed sensor. The practical applicability of sensor probe was also analyzed by detecting the STG from the bacteria present in surface water.
机译：在各种生物样品中检测肠毒素大肠杆菌在人类健康中具有巨大的重要性。在这个方向上，我们设计了一种可用的无电化学生物传感器，用于通过检测ST基因来高度选择性地检测大肠杆菌。使用聚合酶链反应确认传感器探针检测STG的能力。基于固定在筛选印刷碳电极上的铂纳米粒子壳聚糖纳米复合材料上固定的STG特异性探针制备，其特征在于循环伏安法，透射电子显微镜和傅里叶变换红外光谱。通过使用电化学阻抗光谱（EIS）技术分析STG杂交来实现高度敏感的标签。 EIS分析显示STG相互作用在固定在纳米复合膜上的高选择性SSDNA探针后的电荷转移电阻的显着增加。评价电荷转移电阻的增加，以进行不同浓度的STG，其显示1.0×10（-12）和1.0×10（-4）之间的动态范围，检测限为3.6x10（-14）m（RSD <4.5％ ）。还研究了传感器探针的再生，并评估了由于非靶序列引起的干扰，以确保设计传感器的选择性。还通过检测表面水中存在的细菌的STG来分析传感器探针的实际适用性。

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