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首页> 外文期刊>Electroanalysis >Target-stimulated DNAzyme Concatamers Released from Aptasensor for Highly Sensitive and Specific Detection of Progesterone
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Target-stimulated DNAzyme Concatamers Released from Aptasensor for Highly Sensitive and Specific Detection of Progesterone

机译：靶向刺激的二唑粘膜粘附剂，用于高敏感和特异性孕酮的特异性检测

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摘要

Aiming at the detection of ultralow concentration target progesterone (Pro), a novel electrochemical aptasensor based on DNAzyme concatamers signal amplification strategy was proposed. The strategy consists of target DNA strands (TDNAs), and two different hairpin DNA molecules (H1 and H2). The signal is amplified by the large amount of DNAzyme. The TDNAs modified on the electrode open H1 structures in sequence and propagate a reaction of hybridization events between two alternating hairpins (H1and H2) to obtain abundant DNAzyme concatamers. Upon target Pro introduction, a specific Pro-TDNAs reaction was executed, thereby resulting in the release of DNAzyme concatamers from the electrode. Subsequent differential pulse voltammetry(DPV) detection of aminoazobenzene (DAP) resulting by DNAzyme catalyze the oxidation of o-phenylenediamine (OPD) with the aid of hydrogen peroxide (H2O2). Likewise, a small amount of target Pro can efficiently induce the release of a large number of the DNAzyme from the electrode in the form of DNAzyme concatamer. Under optimal conditions, the the proposed assay presents good electrochemical responses for determination of target Pro in the range of 0.5 to 15ng/mL with the detection limit of 0.36ng/mL. In addition, the resulting sensor can successfully distinguish Pro from coexisting interfering substance and show good stability and high repeatability. What's more, the methodology has also been demonstrated by assaying Pro-spiked samples in serum.
机译：旨在检测超级浓度靶孕激素（Pro），提出了一种基于DNAzyme Condishers信号放大策略的新型电化学Aptasensor。该策略由靶DNA链（TDNA）和两种不同的发夹DNA分子（H1和H 2）组成。通过大量的DNazyme扩增该信号。在电极上修饰的TDNA依次打开H1结构，并在两个交替发夹（H1和H 2）之间传播杂交事件的反应，以获得丰富的DNazyme粘附剂。在靶专业介绍后，执行特异性PRO-TDNA反应，从而导致来自电极的DNazyme粘附剂的释放。随后的差分脉冲伏安法（DPV）检测通过DNazyme借助过氧化氢（H2O2）催化O-苯二胺（OPD）的氧化。同样，少量的靶Pro可以有效地以DNazyme Condatamer的形式从电极上有效地诱导大量DNAzyme。在最佳条件下，所提出的测定呈良好的电化学反应，用于测定靶专题的测定在0.5至15ng / ml的范围内，检测限为0.36ng / ml。另外，所得到的传感器可以成功地区分Pro与共存干扰物质，并显示出良好的稳定性和高可重复性。更重要的是，通过测定血清中的纯掺入样品来证明方法。

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