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Graphene/Poly(vinylferrocene) Composite Based Amperometric Biosensor for L-lysine Determination

机译:基于石墨烯/聚(乙烯基加罗乙烯)复合的余量生物传感器,用于L-赖氨酸测定

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摘要

A novel and sensitive amperometric biosensor for L-lysine determination based on a glassy carbon electrode (GCE) modified with graphene (GR) and redox polymer poly(vinylferrocene) (PVF) was constructed. L-lysine--oxidase was immobilized onto the modified GCE by a glutaraldehyde/bovine serum albumin cross-linking procedure. SEM, CV and EIS were used for the characterization of the surface morphology and stepwise fabrication processes of PVF/GR composite. Optimal composition of the biosensor and experimental conditions that affect the performance of the biosensor are discussed. The effect of buffer pH on biosensor response was studied in detail over a wide pH range. L-lysine biosensor displayed a linear range of 9.9x10(-7) - 3.1x10(-4) M with a low detection limit of 2.3x10(-7) M and K-M(app) value of 0.4mM. The L-lysine biosensor was tested using pharmaceutical sample and cheese with satisfactory results.
机译:构建基于用石墨烯(GR)和氧化还原聚合物聚(Vinylferocene)(PVF)改性的玻璃碳电极(GCE)的L-赖氨酸测定的新颖和敏感的敏感的生物传感器。 通过戊二醛/牛血清白蛋白交联程序将L-赖氨酸 - 氧化酶固定在改性的GCE上。 SEM,CV和EIS用于表征PVF / GR复合材料的表面形态和逐步制造方法。 讨论了影响生物传感器性能的生物传感器和实验条件的最佳组成。 在宽的pH范围内详细研究了缓冲液pH对生物传感器响应的影响。 L-赖氨酸生物传感器显示9.9x10(-7) - 3.1x10(-4)m的线性范围,低检测限为2.3x10(-7)m和k-m(app)值为0.4mm。 使用药物样品和奶酪测试L-赖氨酸生物传感器,结果令人满意。

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