Voltammetric Detection of Thrombin by Labeling with Osmium Tetroxide Bipyridine and Binding with Aptamers on a Gold Electrode

Galagedera Sarasi K. K.; Huynh Loan; Wachholz Falko; Jacobsen Martin; Haruehanroengra Phensinee; Sheng Jia; Chen Alan A.; Flechsig Gerd‐Uwe

摘要

Abstract > This communication reports on electrochemical detection of thrombin based on labeling with osmium tetroxide bipyridine [OsO <sub>4</sub> (bipy)]. Tryptophan amino acids can be labeled at the C?C‐double bond, and at least some tryptophan moieties are accessible for labeling in thrombin. Using the catalytic hydrogen signal from adsorptive stripping voltammetry performed on hanging mercury drop electrode, we could detect as little as 1.47?nM [OsO <sub>4</sub> (bipy)]‐modified thrombin. We also tested the binding of [OsO <sub>4</sub> (bipy)]‐modified thrombin with the classic thrombin binding aptamer (TBA) on gold electrodes. This preliminary study revealed that even after modification, a major part of the affinity was conserved, and that the aptamer self‐assembled monolayer (SAM) could be regenerated several times. Molecular simulations confirm that [OsO <sub>4</sub> (bipy)]‐modified thrombin largely preserves the high binding affinity also of the alternative HD22 aptamer to thrombin, albeit at slightly reduced affinities due to steric hindrance when tryptophans 96 and 237 are labelled. Based on these simulations, compensatory modifications in the aptamer should result in significantly improved binding with labelled thrombin. This combined experimental‐computational approach lays the groundwork for the rational design of improved aptamer sensors for analytical applications. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>摘要</ title> >本通信报告凝血酶的电化学检测基于用锇十字酮氧化物Biphyridine的标记[OSO <sub > 4 </ sub>（bipy）]。色氨酸氨基酸可以标记在C = C-C-双键，并且至少一些色氨酸部分可用于在凝血酶中标记。使用来自吸附溶液伏安法在悬垂的汞滴电极上进行的催化氢信号，我们可以检测到1.47Ω·nm [OSO <sub> 4 </ sub>（Bipy）] - 改性凝血酶。我们还测试了[OSO <sub> 4 </ sub>（Bipy）] - 改性凝血酶与金电极的经典凝血酶结合适体（TBA）的结合。这种初步研究表明，即使在修饰后，仍然保守了亲和力的主要部分，并且可以将适体自组装的单层（SAM）几次再生。分子模拟证实[OSO <sub> 4 </ sub>（Bipy）] - 改性凝血酶在很大程度上使替代HD22适体的高结合亲和力保持在凝血酶上，尽管当色氨酸96和237时，由于空间障碍而略微降低的亲和力。被标记为。基于这些模拟，Aptamer中的补偿修饰应导致与标记凝血酶的结合显着改善。这种组合的实验计算方法为分析应用的改进适体传感器的合理设计奠定了基础。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共4页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Galagedera Sarasi K. K.&option=202" target="_blank" rel="nofollow">Galagedera Sarasi K. K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huynh Loan&option=202" target="_blank" rel="nofollow">Huynh Loan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wachholz Falko&option=202" target="_blank" rel="nofollow">Wachholz Falko;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jacobsen Martin&option=202" target="_blank" rel="nofollow">Jacobsen Martin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haruehanroengra Phensinee&option=202" target="_blank" rel="nofollow">Haruehanroengra Phensinee;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sheng Jia&option=202" target="_blank" rel="nofollow">Sheng Jia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Alan A.&option=202" target="_blank" rel="nofollow">Chen Alan A.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Flechsig Gerd‐Uwe&option=202" target="_blank" rel="nofollow">Flechsig Gerd‐Uwe;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistryUniversity at Albany-SUNY1400 Washington Ave Albany NY 12222 United States;</p> <p>Department of ChemistryUniversity at Albany-SUNY1400 Washington Ave Albany NY 12222 United States;</p> <p>Department of ChemistryUniversity of RostockA.-Einstein-Str. 3a D-18055 Rostock Germany;</p> <p>Department of ChemistryUniversity of RostockA.-Einstein-Str. 3a D-18055 Rostock Germany;</p> <p>Department of ChemistryUniversity at Albany-SUNY1400 Washington Ave Albany NY 12222 United States;</p> <p>Department of ChemistryUniversity at Albany-SUNY1400 Washington Ave Albany NY 12222 United States;</p> <p>Department of ChemistryUniversity at Albany-SUNY1400 Washington Ave Albany NY 12222 United States;</p> <p>Department of ChemistryUniversity at Albany-SUNY1400 Washington Ave Albany NY 12222 United States;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hanging mercury drop electrode&option=203" rel="nofollow">Hanging mercury drop electrode;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Osmium tetroxide-2&option=203" rel="nofollow">Osmium tetroxide-2;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=2’-bipyridyl&option=203" rel="nofollow">2’-bipyridyl;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Thrombin binding aptamer&option=203" rel="nofollow">Thrombin binding aptamer;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gold electrodes&option=203" rel="nofollow">Gold electrodes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Adsorptive stripping voltammetry&option=203" rel="nofollow">Adsorptive stripping voltammetry;</a> </p> <div class="translation"> 机译：悬挂的水银滴电极;锇十字颗氧化物-2;2'-双吡啶基;凝血酶结合适体;金电极;吸附剥离伏安法; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023174775.html">Voltammetric Detection of Thrombin by Labeling with Osmium Tetroxide Bipyridine and Binding with Aptamers on a Gold Electrode</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Galagedera Sarasi K. 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