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Electrochemical Signal-Triggered Release of Biomolecules Functionalized with His-tag Units

机译：用HIS标签单元官能化的电化学信号触发的生物分子释放

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摘要

His-tagged molecular species, a ferrocene derivative and Protein A, were immobilized on electrode surfaces (Au and graphite) through formation of a chelated complex in the presence of Cu2+ cations used as bridging units. The complex was cleaved and the attached molecules were released from the electrode surface by applying reductive potential to the electrodes resulting in Cu2+ reduction, thus decomposing the chelate complex. The molecule release process was followed by cyclic voltammetry in case of the ferrocene derivative. His-tagged Protein A was additionally labeled with a fluorescent tag and its release was followed by fluorescence measurements in the solution and by impedance spectroscopy at the electrode. The studied release of the His-tagged redox species and biomolecules was considered as a new generic approach to the signal-controlled molecule release applicable in various biotechnological and biomedical applications.
机译：His标记的分子种，二茂铁衍生物和蛋白A，通过在用作桥接单元的Cu 2 +阳离子的情况下形成螯合的络合物，在电极表面（Au和石墨）上固定在电极表面（au和石墨）上。 将复合物裂解，通过向电极施加还原电位，从电极表面释放附着的分子，从而导致Cu 2 +降低，从而将螯合物复合物分解。 在二茂铁衍生物的情况下，分子释放过程之后是环状伏安法。 他标记的蛋白质A另外用荧光标签标记，然后在溶液中荧光测量和电极处的阻抗光谱。 研究其标记的氧化还原物种和生物分子的研究被认为是一种新的通用方法，用于适用于各种生物技术和生物医学应用的信号控制分子释放。

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